Koleobacter methoxysyntrophicus gen. nov., sp. nov., a novel anaerobic bacterium isolated from deep subsurface oil field and proposal of Koleobacteraceae fam. nov. and Koleobacterales ord. nov. within the class Clostridia of the phylum Firmicutes.

Autor: Sakamoto S; Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; JST ERATO Nomura Microbial Community Control Project, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan., Nobu MK; Bioproduction Research Institute, AIST, 1-1-1 Higashi, Tsukuba 305-8566, Japan. Electronic address: m.nobu@aist.go.jp., Mayumi D; Institute for Geo-Resources and Environment, Geological Survey of Japan, AIST, 1-1-1, Higashi, Tsukuba 305-8566, Ibaraki, Japan., Tamazawa S; Bioproduction Research Institute, AIST, 1-1-1 Higashi, Tsukuba 305-8566, Japan; Northern Advancement Center for Science & Technology, H-RISE, 5-3 Sakae-machi, Horonobe-cho, Teshio-gun, BPRI, Hokkaido 098-3221, Japan., Kusada H; JST ERATO Nomura Microbial Community Control Project, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; Bioproduction Research Institute, AIST, 1-1-1 Higashi, Tsukuba 305-8566, Japan., Yonebayashi H; Technical Research Center, INPEX CORPORATION, 9-23-30, Kitakarasuyama, Setagaya, 157-0061, Tokyo, Japan., Iwama H; Technical Research Center, INPEX CORPORATION, 9-23-30, Kitakarasuyama, Setagaya, 157-0061, Tokyo, Japan., Ikarashi M; Technical Research Center, INPEX CORPORATION, 9-23-30, Kitakarasuyama, Setagaya, 157-0061, Tokyo, Japan., Wakayama T; Technical Research Center, INPEX CORPORATION, 9-23-30, Kitakarasuyama, Setagaya, 157-0061, Tokyo, Japan., Maeda H; Institute for Geo-Resources and Environment, Geological Survey of Japan, AIST, 1-1-1, Higashi, Tsukuba 305-8566, Ibaraki, Japan; Technical Research Center, INPEX CORPORATION, 9-23-30, Kitakarasuyama, Setagaya, 157-0061, Tokyo, Japan., Sakata S; Institute for Geo-Resources and Environment, Geological Survey of Japan, AIST, 1-1-1, Higashi, Tsukuba 305-8566, Ibaraki, Japan., Tamura T; Bioproduction Research Institute, AIST, 1-1-1 Higashi, Tsukuba 305-8566, Japan; Computational Bio Big-Data Open Innovation Laboratory (CBBD-OIL), AIST, 3-4-1 Okubo, Shinjuku, Tokyo 169-8555, Japan., Nomura N; Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; JST ERATO Nomura Microbial Community Control Project, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan., Kamagata Y; Bioproduction Research Institute, AIST, 1-1-1 Higashi, Tsukuba 305-8566, Japan., Tamaki H; Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; JST ERATO Nomura Microbial Community Control Project, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan; Bioproduction Research Institute, AIST, 1-1-1 Higashi, Tsukuba 305-8566, Japan. Electronic address: tamaki-hideyuki@aist.go.jp.
Jazyk: angličtina
Zdroj: Systematic and applied microbiology [Syst Appl Microbiol] 2021 Jan; Vol. 44 (1), pp. 126154. Date of Electronic Publication: 2020 Nov 10.
DOI: 10.1016/j.syapm.2020.126154
Abstrakt: An anaerobic thermophilic, rod-shaped bacterium possessing a unique non-lipid sheathed-like structure enveloping a single-membraned cell, designated strain NRmbB1 T was isolated from at the deep subsurface oil field located in Yamagata Prefecture, Japan. Growth occurred with 40-60°C (optimum, 55°C), 0-2% (2%), NaCl and pH 6.0-8.5 (8.0). Fermentative growth with various sugars was observed. Glucose-grown cells generated acetate, hydrogen, pyruvate and lactate as the main end products. Syntrophic growth occurred with glucose, pyruvate and 3,4,5-trimethoxybenzoate in the presence of an H 2 -scavenging partner, and growth on 3,4,5-trimethoxybenzoate was only observed under syntrophic condition. The predominant cellular fatty acids were C 16:0, iso-C 16:0 , anteiso-C 15:0 , and iso-C 14:0 . Respiratory quinone was not detected. The genomic G+C content was 40.8mol%. Based on 16S rRNA gene phylogeny, strain NRmbB1 T belongs to a distinct order-level clade in the class Clostridia of the phylum Firmicutes, sharing low similarity with other isolated organisms (i.e., 87.5% for top hit Moorella thermoacetica DSM 2955 T ). In total, chemotaxonomic, phylogenetic and genomic characterization revealed that strain NRmbB1 T (=KCTC 25035 T , =JCM 39120 T ) represents a novel species of a new genus. In addition, we also propose the associated family and order as Koleobacteraceae fam. nov and Koleobacterales ord. nov., respectively.
(Copyright © 2020. Published by Elsevier GmbH.)
Databáze: MEDLINE
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