Untargeted metabolomic and molecular network approaches to reveal tomato root secondary metabolites.

Autor: Messaili S; Institut de Chimie Organique et Analytique, Université d'Orléans, CNRS, UMR 7311, BP 6759, Orléans, France., Qu Y; Université Côte d'Azur, INRAe, CNRS, Institut Sophia Agrobiotech, UMR 1355-7254, 06903 Sophia Antipolis, France., Fougère L; Institut de Chimie Organique et Analytique, Université d'Orléans, CNRS, UMR 7311, BP 6759, Orléans, France., Colas C; Institut de Chimie Organique et Analytique, Université d'Orléans, CNRS, UMR 7311, BP 6759, Orléans, France.; Centre de Biophysique Moléculaire, CNRS, Université d'Orléans, UPR 4311, Orléans, France., Desneux N; Université Côte d'Azur, INRAe, CNRS, Institut Sophia Agrobiotech, UMR 1355-7254, 06903 Sophia Antipolis, France., Lavoir AV; Université Côte d'Azur, INRAe, CNRS, Institut Sophia Agrobiotech, UMR 1355-7254, 06903 Sophia Antipolis, France., Destandau E; Institut de Chimie Organique et Analytique, Université d'Orléans, CNRS, UMR 7311, BP 6759, Orléans, France., Michel T; Université Côte d'Azur, CNRS, Institut de Chimie de Nice, UMR 7272, Nice, France.
Jazyk: angličtina
Zdroj: Phytochemical analysis : PCA [Phytochem Anal] 2021 Sep; Vol. 32 (5), pp. 672-684. Date of Electronic Publication: 2020 Nov 22.
DOI: 10.1002/pca.3014
Abstrakt: Introduction: The tomato plant, Solanum lycopersicum L. (Solanaceae), is one of the most widely consumed vegetables in the world and plays an important role in human diet. Tomato cultivars are hosts for diverse types of pests, implying diverse chemical defence strategies. Glycoalkaloids are the main specialised metabolites produced by tomato leaves and fruits to protect against pests. However, the roots have received little attention, leading to limited knowledge about their phytochemical content.
Objective: The main goal of the current study was the development of an untargeted ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) based metabolomic approach to study phytochemical variations in tomato roots at two different development stages (i.e. 34th and 62nd day after sowing).
Methods: UHPLC-HRMS was used to establish the fingerprint of 24 batches of tomato roots. Statistical analyses were performed to highlight the compounds that discriminated between young and mature tomato roots. A dereplication strategy using molecular networking and HRMS/MS data was set up to identify the metabolites regulated during early root development.
Key Findings: The main biomarkers were guanidine and adenosine derivatives associated with tryptophan. Secondary metabolites such as glycoalkaloids and steroidal alkaloids were also characterised. Most of the metabolites were up-regulated in young tomato roots (34 days old) while tryptophan was up-regulated in the older roots (62 days old).
Conclusion: The metabolic changes observed in this work contribute to a deeper understanding of early-stage root development and may help our understanding of the complex processes involved in the tomato root defence arsenal.
(© 2020 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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