The Human Leukocyte Antigen Class II Immunopeptidome of the SARS-CoV-2 Spike Glycoprotein.

Autor:	Knierman MD; Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA., Lannan MB; Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA., Spindler LJ; Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA., McMillian CL; Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA., Konrad RJ; Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA., Siegel RW; Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA. Electronic address: siegel_robert@lilly.com.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2020 Dec 01; Vol. 33 (9), pp. 108454. Date of Electronic Publication: 2020 Nov 13.
DOI:	10.1016/j.celrep.2020.108454
Abstrakt:	Precise elucidation of the antigen sequences for T cell immunosurveillance greatly enhances our ability to understand and modulate humoral responses to viral infection or active immunization. Mass spectrometry is used to identify 526 unique sequences from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein extracellular domain in a complex with human leukocyte antigen class II molecules on antigen-presenting cells from a panel of healthy donors selected to represent a majority of allele usage from this highly polymorphic molecule. The identified sequences span the entire spike protein, and several sequences are isolated from a majority of the sampled donors, indicating promiscuous binding. Importantly, many peptides derived from the receptor binding domain used for cell entry are identified. This work represents a precise and comprehensive immunopeptidomic investigation with the SARS-CoV-2 spike glycoprotein and allows detailed analysis of features that may aid vaccine development to end the current coronavirus disease 2019 (COVID-19) pandemic. Competing Interests: Declaration of Interests The authors declare no competing interests. (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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