Streptococcus agalactiae clinical isolates in Northwest Iran: antibiotic susceptibility, molecular typing, and biofilm formation.

Autor:	Shadbad MA; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran., Kafil HS; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Rezaee MA; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Farzami MR; Reference Health Laboratory, Ministry of Health & Medical Education, Tehran, Iran., Dehkharghani AD; Reference Health Laboratory, Ministry of Health & Medical Education, Tehran, Iran., Sadeghi J; Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Gholizadeh P; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Khodaei F; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Aghazadeh M; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Jazyk:	angličtina
Zdroj:	GMS hygiene and infection control [GMS Hyg Infect Control] 2020 Sep 29; Vol. 15, pp. Doc23. Date of Electronic Publication: 2020 Sep 29 (Print Publication: 2020).
DOI:	10.3205/dgkh000358
Abstrakt:	Background: Group B Streptococcus ( S. agalactiae ) is one of the colonizing bacteria in pregnant women which can be a causative agent of meningitis and neonatal sepsis. This organism has also been increasingly related to invasive infections in non-pregnant adults. Objective: In present study, we aimed to characterize the clonality of biofilm-producing S. agalactiae isolates from various sources from two different clinical laboratories in Tehran, Iran. Materials and Methods: S. agalactiae isolates were collected from community-acquired (CA) and hospital-acquired (HA) infections in pregnant and non-pregnant adults. The antimicrobial susceptibility patterns and biofilm formation ability were determined. In addition, pulse field gel electrophoresis (PFGE) was used to verify the clonal diversity of isolates. Results: Out of the 87 isolates, 15 (16.6%) formed biofilm. The antibiotic resistance rate was 98.85% for clindamycin, 98.85% for tetracycline, followed by 29.88% for erythromycin, 9.19% for moxifloxacin and 6.89% for levofloxacin. The PFGE patterns revealed a total of 16 different clusters consisting of 6 single types (STs). Conclusion: This study evaluated the biofilm formation of clinical S. agalactiae , which may be a step towards understanding its role in pathological processes. Biofilm formation was significant only in the hypervirulent ST-17 clone. Intraclonal spread of isolates indicates that a local lineage of isolates is responsible for infection by these bacteria. Competing Interests: The authors declare that they have no competing interests. (Copyright © 2020 Shadbad et al.)
Databáze:	MEDLINE
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