A network of transcriptional repressors modulates auxin responses.

Autor: Truskina J; Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Lyon, France.; School of Biosciences, University of Nottingham, Loughborough, UK., Han J; School of Biosciences, University of Nottingham, Loughborough, UK., Chrysanthou E; School of Biosciences, University of Nottingham, Loughborough, UK., Galvan-Ampudia CS; Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Lyon, France., Lainé S; Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Lyon, France., Brunoud G; Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Lyon, France., Macé J; Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Lyon, France., Bellows S; School of Mathematical Sciences, University of Nottingham, Nottingham, UK., Legrand J; Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Lyon, France., Bågman AM; Department of Plant Biology, University of California Davis, Davis, CA, USA.; Genome Center, University of California Davis, Davis, CA, USA., Smit ME; Department of Plant Biology, University of California Davis, Davis, CA, USA.; Genome Center, University of California Davis, Davis, CA, USA., Smetana O; Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland.; Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland., Stigliani A; Université Grenoble Alpes, CNRS, CEA, INRAE, IRIG-DBSCI-LPCV, Grenoble, France., Porco S; School of Biosciences, University of Nottingham, Loughborough, UK., Bennett MJ; School of Biosciences, University of Nottingham, Loughborough, UK., Mähönen AP; Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland.; Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland., Parcy F; Université Grenoble Alpes, CNRS, CEA, INRAE, IRIG-DBSCI-LPCV, Grenoble, France., Farcot E; School of Biosciences, University of Nottingham, Loughborough, UK.; School of Mathematical Sciences, University of Nottingham, Nottingham, UK., Roudier F; Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Lyon, France., Brady SM; Department of Plant Biology, University of California Davis, Davis, CA, USA.; Genome Center, University of California Davis, Davis, CA, USA., Bishopp A; School of Biosciences, University of Nottingham, Loughborough, UK. Anthony.Bishopp@nottingham.ac.uk., Vernoux T; Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Lyon, France. teva.vernoux@ens-lyon.fr.
Jazyk: angličtina
Zdroj: Nature [Nature] 2021 Jan; Vol. 589 (7840), pp. 116-119. Date of Electronic Publication: 2020 Nov 18.
DOI: 10.1038/s41586-020-2940-2
Abstrakt: The regulation of signalling capacity, combined with the spatiotemporal distribution of developmental signals themselves, is pivotal in setting developmental responses in both plants and animals 1 . The hormone auxin is a key signal for plant growth and development that acts through the AUXIN RESPONSE FACTOR (ARF) transcription factors 2-4 . A subset of these, the conserved class A ARFs 5 , are transcriptional activators of auxin-responsive target genes that are essential for regulating auxin signalling throughout the plant lifecycle 2,3 . Although class A ARFs have tissue-specific expression patterns, how their expression is regulated is unknown. Here we show, by investigating chromatin modifications and accessibility, that loci encoding these proteins are constitutively open for transcription. Through yeast one-hybrid screening, we identify the transcriptional regulators of the genes encoding class A ARFs from Arabidopsis thaliana and demonstrate that each gene is controlled by specific sets of transcriptional regulators. Transient transformation assays and expression analyses in mutants reveal that, in planta, the majority of these regulators repress the transcription of genes encoding class A ARFs. These observations support a scenario in which the default configuration of open chromatin enables a network of transcriptional repressors to regulate expression levels of class A ARF proteins and modulate auxin signalling output throughout development.
Databáze: MEDLINE
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