PRC1 is a critical regulator for anaphase spindle midzone assembly and cytokinesis in mouse oocyte meiosis.

Autor: Li XH; College of Animal Science and Technology, Nanjing Agricultural University, China., Ju JQ; College of Animal Science and Technology, Nanjing Agricultural University, China., Pan ZN; College of Animal Science and Technology, Nanjing Agricultural University, China., Wang HH; College of Animal Science and Technology, Nanjing Agricultural University, China., Wan X; College of Animal Science and Technology, Nanjing Agricultural University, China., Pan MH; College of Animal Science and Technology, Nanjing Agricultural University, China., Xu Y; College of Animal Science and Technology, Nanjing Agricultural University, China., Sun MH; College of Animal Science and Technology, Nanjing Agricultural University, China., Sun SC; College of Animal Science and Technology, Nanjing Agricultural University, China.
Jazyk: angličtina
Zdroj: The FEBS journal [FEBS J] 2021 May; Vol. 288 (9), pp. 3055-3067. Date of Electronic Publication: 2020 Nov 30.
DOI: 10.1111/febs.15634
Abstrakt: Protein regulator of cytokinesis 1 (PRC1) is a microtubule bundling protein that is involved in the regulation of the central spindle bundle and spindle orientation during mitosis. However, the functions of PRC1 during meiosis have rarely been studied. In this study, we explored the roles of PRC1 during meiosis using an oocyte model. Our results found that PRC1 was expressed at all stages of mouse oocyte meiosis, and PRC1 accumulated in the midzone/midbody during anaphase/telophase I. Moreover, depleting PRC1 caused defects in polar body extrusion during mouse oocyte maturation. Further analysis found that PRC1 knockdown did not affect meiotic spindle formation or chromosome segregation; however, deleting PRC1 prevented formation of the midzone and midbody at the anaphase/telophase stage of meiosis I, which caused cytokinesis defects and further induced the formation of two spindles in the oocytes. PRC1 knockdown increased the level of tubulin acetylation, indicating that microtubule stability was affected. Furthermore, KIF4A and PRC1 showed similar localization in the midzone/midbody of oocytes at anaphase/telophase I, while the depletion of KIF4A affected the expression and localization of PRC1. The PRC1 mRNA injection rescued the defects caused by PRC1 knockdown in oocytes. In summary, our results suggest that PRC1 is critical for midzone/midbody formation and cytokinesis under regulation of KIF4A in mouse oocytes.
(© 2020 Federation of European Biochemical Societies.)
Databáze: MEDLINE
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