A simplified design for the C. elegans lifespan machine.

Autor:	Abbott M; Rutgers University, Department of Molecular Biology and Biochemistry, Piscataway, NJ, 08854, USA., Banse SA; Institute of Ecology and Evolution, University of Oregon, Eugene, OR 97403, USA., Melentijevic I; Rutgers University, Department of Molecular Biology and Biochemistry, Piscataway, NJ, 08854, USA., Jarrett CM; Institute of Ecology and Evolution, University of Oregon, Eugene, OR 97403, USA., Ange JS; Rutgers University, Department of Molecular Biology and Biochemistry, Piscataway, NJ, 08854, USA., Sedore CA; Institute of Ecology and Evolution, University of Oregon, Eugene, OR 97403, USA., Falkowski R; Rutgers University, Department of Molecular Biology and Biochemistry, Piscataway, NJ, 08854, USA., Blue BW; Institute of Ecology and Evolution, University of Oregon, Eugene, OR 97403, USA., Coleman-Hulbert AL; Institute of Ecology and Evolution, University of Oregon, Eugene, OR 97403, USA., Johnson E; Institute of Ecology and Evolution, University of Oregon, Eugene, OR 97403, USA., Guo M; Division of Aging Biology, National Institute on Aging, Bethesda, MD, 20892, USA., Lithgow GJ; The Buck Institute for Research on Aging, Novato, CA, 94945, USA., Phillips PC; Institute of Ecology and Evolution, University of Oregon, Eugene, OR 97403, USA., Driscoll M; Rutgers University, Department of Molecular Biology and Biochemistry, Piscataway, NJ, 08854, USA.
Jazyk:	angličtina
Zdroj:	Journal of biological methods [J Biol Methods] 2020 Oct 26; Vol. 7 (4), pp. e137. Date of Electronic Publication: 2020 Oct 26 (Print Publication: 2020).
DOI:	10.14440/jbm.2020.332
Abstrakt:	Caenorhabditis elegans ( C. elegans ) lifespan assays constitute a broadly used approach for investigating the fundamental biology of longevity. Traditional C. elegans lifespan assays require labor-intensive microscopic monitoring of individual animals to evaluate life/death over a period of weeks, making large-scale high throughput studies impractical. The lifespan machine developed by Stroustrup et al . (2013) adapted flatbed scanner technologies to contribute a major technical advance in the efficiency of C. elegans survival assays. Introducing a platform in which large portions of a lifespan assay are automated enabled longevity studies of a scope not possible with previous exclusively manual assays and facilitated novel discovery. Still, as initially described, constructing and operating scanner-based lifespan machines requires considerable effort and expertise. Here we report on design modifications that simplify construction, decrease cost, eliminate certain mechanical failures, and decrease assay workload requirements. The modifications we document should make the lifespan machine more accessible to interested laboratories. Competing Interests: Competing interests: The authors have declared that no competing interests exist. (© 2013-2020 The Journal of Biological Methods, All rights reserved.)
Databáze:	MEDLINE
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