| Autor: |
Deng Y; Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China., Xu Y; Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China., Xu S; Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China., Zhang Y; Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China., Han B; Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China., Liu Z; Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China., Liu X; Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China., Zhu Z; Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China. |
| Abstrakt: |
This study aimed to identify long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) differentially expressed (DE) during keloid formation, predict DElncRNA-DEmiRNA-DEmRNA interactions, and construct a competing endogenous RNA (ceRNA) network through secondary data mining of keloid-related sequencing and microarray data in the open-source Gene Expression Omnibus (GEO) database. The GSE113621 dataset was downloaded from the GEO database, |log 2 FC|>1 and p<0.05 were set as screening criteria, genes expressed only in keloid-prone individuals were selected as research objects, and DEmRNAs, DElncRNAs, and DEmiRNAs before injury and 6 weeks after injury were screened. A Pearson correlation coefficient (PCC) of > 0.95 was selected as the index to predict the targeting relationships among lncRNAs, miRNAs, and mRNAs; and a network diagram was constructed using Cytoscape. The expression of 2356 lncRNAs was changed in the keloid-prone group-1306 were upregulated and 1050 were downregulated. Six lncRNAs, namely, 2 upregulated (DLEU2 and AP000317.2) and 4 downregulated (ADIRF-AS1, AC006333.2, AL137127.1 and LINC01725) lncRNAs, were expressed only in the keloid-prone group and were used to construct a ceRNA network. DLEU2 may regulate fibroblast proliferation, differentiation, and apoptosis through hsa-miR-30a-5p/hsa-miR-30b-5p. In-depth mining of GEO data indicated that lncRNAs and a ceRNA regulatory network participate in the wound healing process in keloid-prone individuals, possibly providing novel intervention targets and treatment options for keloid scars. |
