Discovery of novel pathways for carbohydrate metabolism.

Autor: Stack TMM; Carl. R. Woese Institute for Genomic Biology, University of Illinois, Urbana, IL 61801, United States., Gerlt JA; Carl. R. Woese Institute for Genomic Biology, University of Illinois, Urbana, IL 61801, United States; Departments of Biochemistry and Chemistry, University of Illinois, Urbana, IL 61801, United States. Electronic address: j-gerlt@igb.illinois.edu.
Jazyk: angličtina
Zdroj: Current opinion in chemical biology [Curr Opin Chem Biol] 2021 Apr; Vol. 61, pp. 63-70. Date of Electronic Publication: 2020 Nov 13.
DOI: 10.1016/j.cbpa.2020.09.005
Abstrakt: Closing the gap between the increasing availability of complete genome sequences and the discovery of novel enzymes in novel metabolic pathways is a significant challenge. Here, we review recent examples of assignment of in vitro enzymatic activities and in vivo metabolic functions to uncharacterized proteins, with a focus on enzymes and metabolic pathways involved in the catabolism and biosynthesis of monosaccharides and polysaccharides. The most effective approaches are based on analyses of sequence-function space in protein families that provide clues for the predictions of the functions of the uncharacterized enzymes. As summarized in this Opinion, this approach allows the discovery of the catabolism of new molecules, new pathways for common molecules, and new enzymatic chemistries.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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