Cellular and Functional Physiopathology of Bull Sperm With Altered Sperm Freezability.

Autor: Hitit M; Department of Animal and Dairy Sciences, Mississippi State University, Starkville, MS, United States.; Department of Animal Genetics, Kastamonu University, Kastamonu, Turkey., Ugur MR; Department of Animal and Dairy Sciences, Mississippi State University, Starkville, MS, United States., Dinh TTN; Department of Animal and Dairy Sciences, Mississippi State University, Starkville, MS, United States., Sajeev D; Department of Animal and Dairy Sciences, Mississippi State University, Starkville, MS, United States., Kaya A; Department of Reproduction and Artificial Insemination, Selcuk University, Konya, Turkey., Topper E; URUS Group LP, Madison, WI, United States., Tan W; Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Starkville, MS, United States., Memili E; Department of Animal and Dairy Sciences, Mississippi State University, Starkville, MS, United States.
Jazyk: angličtina
Zdroj: Frontiers in veterinary science [Front Vet Sci] 2020 Oct 23; Vol. 7, pp. 581137. Date of Electronic Publication: 2020 Oct 23 (Print Publication: 2020).
DOI: 10.3389/fvets.2020.581137
Abstrakt: The objective of this study was to ascertain the cellular and functional parameters as well as ROS related changes in sperm from bulls with varied sperm freezability phenotypes. Using principal component analysis (PCA), the variables were reduced to two principal components, of which PC1 explained 48% of the variance, and PC2 explained 24% of the variance, and clustered animals into two distinct groups of good freezability (GF) and poor freezability (PF). In ROS associated pathophysiology, there were more dead superoxide anion positive (Dead SO+) sperm in GF bulls than those in PF (15.72 and 12.00%; P = 0.024), and that Dead SO+ and live hydrogen positive cells (live H 2 O 2 +) were positively correlated with freezability, respectively ( R 2 = 0.55, P < 0.0130) and (r s = 0.63, P = 0.0498). Related to sperm functional integrity, sperm from PF bulls had greater dead intact acrosome (DIAC) than those from GF bulls (26.29 and 16.10%; P = 0.028) whereas sperm from GF bulls tended to have greater live intact acrosome (LIAC) than those from PF bulls (64.47 and 50.05%; P = 0.084). Sperm with dead reacted acrosome (DRAC) in PF bulls were greater compared to those in GF (19.27 and 11.48%; P = 0.007). While DIAC ( R 2 = 0.56, P = 0.0124) and DRAC ( R 2 = 0.57, P < 0.0111) were negatively correlated with freezability phenotype, LIAC ( R 2 = 0.36, P = 0.0628) was positively correlated. Protamine deficiency (PRM) was similar between sperm from GF and PF bulls (7.20 and 0.64%; P = 0.206) and (r s = 0.70, P = 0.0251) was correlated with freezability. Sperm characteristics associated with cryotolerance are important for advancing both fundamental andrology and assisted reproductive technologies across mammals.
(Copyright © 2020 Hitit, Ugur, Dinh, Sajeev, Kaya, Topper, Tan and Memili.)
Databáze: MEDLINE
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