Development of a Semi-Automated, Bulk Seeding Device for Large Animal Model Implantation of Tissue Engineered Vascular Grafts.

Autor:	Cunnane EM; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United States.; Tissue Engineering Research Group, Department of Anatomy, Royal College of Surgeons in Ireland, Dublin, Ireland., Lorentz KL; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United States., Soletti L; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United States., Ramaswamy AK; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United States., Chung TK; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United States., Haskett DG; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, United States., Luketich SK; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, United States., Tzeng E; Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States., D'Amore A; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, United States.; RiMED Foundation, Palermo, Italy., Wagner WR; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United States.; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, United States.; Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.; Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, United States., Weinbaum JS; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United States.; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, United States.; Department of Pathology, University of Pittsburgh, Pittsburgh, PA, United States., Vorp DA; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, United States.; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, United States.; Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.; Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, United States.; Department of Cardiothoracic Surgery, University of Pittsburgh, Pittsburgh, PA, United States.; Clinical and Translational Sciences Institute, University of Pittsburgh, Pittsburgh, PA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2020 Oct 23; Vol. 8, pp. 597847. Date of Electronic Publication: 2020 Oct 23 (Print Publication: 2020).
DOI:	10.3389/fbioe.2020.597847
Abstrakt:	Vascular tissue engineering is a field of regenerative medicine that restores tissue function to defective sections of the vascular network by bypass or replacement with a tubular, engineered graft. The tissue engineered vascular graft (TEVG) is comprised of a biodegradable scaffold, often combined with cells to prevent acute thrombosis and initiate scaffold remodeling. Cells are most effectively incorporated into scaffolds using bulk seeding techniques. While our group has been successful in uniform, rapid, bulk cell seeding of scaffolds for TEVG testing in small animals using our well-validated rotational vacuum technology, this approach was not directly translatable to large scaffolds, such as those required for large animal testing or human implants. The objective of this study was to develop and validate a semi-automated cell seeding device that allows for uniform, rapid, bulk seeding of large scaffolds for the fabrication of TEVGs appropriately sized for testing in large animals and eventual translation to humans. Validation of our device revealed successful seeding of cells throughout the length of our tubular scaffolds with homogenous longitudinal and circumferential cell distribution. To demonstrate the utility of this device, we implanted a cell seeded scaffold as a carotid interposition graft in a sheep model for 10 weeks. Graft remodeling was demonstrated upon explant analysis using histological staining and mechanical characterization. We conclude from this work that our semi-automated, rotational vacuum seeding device can successfully seed porous tubular scaffolds suitable for implantation in large animals and provides a platform that can be readily adapted for eventual human use. (Copyright © 2020 Cunnane, Lorentz, Soletti, Ramaswamy, Chung, Haskett, Luketich, Tzeng, D’Amore, Wagner, Weinbaum and Vorp.)
Databáze:	MEDLINE
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