Various Adjuvants Effect on Immunogenicity of Puumala Virus Vaccine.

Autor:	Kurashova SS; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow, Russia., Ishmukhametov AA; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow, Russia.; Institute for Translatonal Medicine and Bionechnology, Sechenov First Moscow State Medical University, Moscow, Russia., Dzagurova TK; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow, Russia., Egorova MS; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow, Russia., Balovneva MV; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow, Russia., Nikitin NA; Department of Virology, Lomonosov Moscow State University, Moscow, Russia., Evtushenko EA; Department of Virology, Lomonosov Moscow State University, Moscow, Russia., Karpova OV; Department of Virology, Lomonosov Moscow State University, Moscow, Russia., Markina AA; National Research Center - Institute of Immunology Federal Medical-Biological Agency of Russia, Moscow, Russia., Aparin PG; National Research Center - Institute of Immunology Federal Medical-Biological Agency of Russia, Moscow, Russia., Tkachenko PE; Department of Internal Medicine Propaedeutics, Sechenov First Moscow State Medical University, Moscow, Russia., L Vov VL; National Research Center - Institute of Immunology Federal Medical-Biological Agency of Russia, Moscow, Russia., Tkachenko EA; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2020 Oct 26; Vol. 10, pp. 545371. Date of Electronic Publication: 2020 Oct 26 (Print Publication: 2020).
DOI:	10.3389/fcimb.2020.545371
Abstrakt:	Various adjuvant effects on the immunogenicity of the candidate inactivated Puumala virus vaccine were detected in BALB/c mice. Adjuvants under study were: aluminum hydroxide, spherical particles of Tobacco mosaic virus coat protein, B subunit of heat-labile enterotoxin of Escherichia coli , and low endotoxic lipopolysaccharide of Shigella sonnei . Aluminum hydroxide (1 mg/ml) did not affect neutralizing antibodies' induction and vaccine stability during storage compared to immunization with the vaccine without adjuvant. B subunit of heat-labile enterotoxin (0.2 µg/ml), low endotoxic lipopolysaccharide (50 µg/ml), and plant virus-based spherical particles (300 µg/ml) significantly enhance the humoral immune response of vaccine (p < 0.0001). Pronounced stimulation of IL-12 and IFN- ɣ was observed when mice were immunized with vaccines both with adjuvants (except of aluminum hydroxide) and without adjuvants. It has been shown that low endotoxic lipopolysaccharide contributes not only to enhance the immune response but also to stabilize vaccine immunogenicity during at least 1 year storage. (Copyright © 2020 Kurashova, Ishmukhametov, Dzagurova, Egorova, Balovneva, Nikitin, Evtushenko, Karpova, Markina, Aparin, Tkachenko, L`vov and Tkachenko.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu