Extended Set of GoldenBraid Compatible Vectors for Fast Assembly of Multigenic Constructs and Their Use to Create Geminiviral Expression Vectors.

Autor:	Dusek J; Laboratory of Virology, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia.; Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czechia., Plchova H; Laboratory of Virology, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia., Cerovska N; Laboratory of Virology, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia., Poborilova Z; Laboratory of Virology, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia., Navratil O; Laboratory of Virology, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia., Kratochvilova K; Laboratory of Virology, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia.; Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czechia., Gunter C; Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa., Jacobs R; Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa., Hitzeroth II; Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa., Rybicki EP; Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa., Moravec T; Laboratory of Virology, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2020 Oct 22; Vol. 11, pp. 522059. Date of Electronic Publication: 2020 Oct 22 (Print Publication: 2020).
DOI:	10.3389/fpls.2020.522059
Abstrakt:	Methods for simple and fast assembly of exchangeable standard DNA parts using Type II S restriction enzymes are becoming more and more popular in plant synthetic and molecular biology. These methods enable routine construction of large and complex multigene DNA structures. Two available frameworks emphasize either high cloning capacity (Modular Cloning, MoClo) or simplicity (GoldenBraid, GB). Here we present a set of novel α-level plasmids compatible with the GB convention that extend the ability of GB to rapidly assemble more complex genetic constructs, while maintaining compatibility with all existing GB parts as well as most MoClo parts and GB modules. With the use of our new plasmids, standard GB parts can be assembled into complex assemblies containing 1, 5, 10 and up to theoretically 50 units in each successive level of infinite loop assembly. Assembled DNA constructs can be also combined with conventional binary GB-assemblies (1, 2, 4, 8… units). We demonstrate the usefulness of our framework on single tube assembly of replicating plant expression constructs based on the geminivirus Bean yellow dwarf virus (BeYDV). (Copyright © 2020 Dusek, Plchova, Cerovska, Poborilova, Navratil, Kratochvilova, Gunter, Jacobs, Hitzeroth, Rybicki and Moravec.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu