Cell wall modification by the xyloglucan endotransglucosylase/hydrolase XTH19 influences freezing tolerance after cold and sub-zero acclimation.

Autor: Takahashi D; Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany.; Graduate School of Science & Engineering, Saitama University, Saitama City, Saitama., Johnson KL; La Trobe Institute for Agriculture and Food, La Trobe University, Bundoora, Victoria, Australia.; Sino-Australian Plant Cell Wall Research Centre, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, China., Hao P; La Trobe Institute for Agriculture and Food, La Trobe University, Bundoora, Victoria, Australia.; Sino-Australian Plant Cell Wall Research Centre, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, China., Tuong T; USDA and Department of Crop Science, North Carolina State University, Raleigh, North Carolina, USA., Erban A; Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany., Sampathkumar A; Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany., Bacic A; La Trobe Institute for Agriculture and Food, La Trobe University, Bundoora, Victoria, Australia.; Sino-Australian Plant Cell Wall Research Centre, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, China., Livingston DP 3rd; USDA and Department of Crop Science, North Carolina State University, Raleigh, North Carolina, USA., Kopka J; Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany., Kuroha T; Graduate School of Life Sciences, Tohoku University, Sendai, Japan.; Division of Applied Genetics, Institute of Agrobiological Sciences, National Agriculture and Food Organization (NARO), Tsukuba, Ibaraki, Japan., Yokoyama R; Graduate School of Life Sciences, Tohoku University, Sendai, Japan., Nishitani K; Graduate School of Life Sciences, Tohoku University, Sendai, Japan.; Faculty of Science, Kanagawa University, Hiratsuka, Japan., Zuther E; Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany., Hincha DK; Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2021 Mar; Vol. 44 (3), pp. 915-930. Date of Electronic Publication: 2020 Dec 02.
DOI: 10.1111/pce.13953
Abstrakt: Freezing triggers extracellular ice formation leading to cell dehydration and deformation during a freeze-thaw cycle. Many plant species increase their freezing tolerance during exposure to low, non-freezing temperatures, a process termed cold acclimation. In addition, exposure to mild freezing temperatures after cold acclimation evokes a further increase in freezing tolerance (sub-zero acclimation). Previous transcriptome and proteome analyses indicate that cell wall remodelling may be particularly important for sub-zero acclimation. In the present study, we used a combination of immunohistochemical, chemical and spectroscopic analyses to characterize the cell walls of Arabidopsis thaliana and characterized a mutant in the XTH19 gene, encoding a xyloglucan endotransglucosylase/hydrolase (XTH). The mutant showed reduced freezing tolerance after both cold and sub-zero acclimation, compared to the Col-0 wild type, which was associated with differences in cell wall composition and structure. Most strikingly, immunohistochemistry in combination with 3D reconstruction of centres of rosette indicated that epitopes of the xyloglucan-specific antibody LM25 were highly abundant in the vasculature of Col-0 plants after sub-zero acclimation but absent in the XTH19 mutant. Taken together, our data shed new light on the potential roles of cell wall remodelling for the increased freezing tolerance observed after low temperature acclimation.
(© 2020 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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