Development of thermosensitive resiquimod-loaded liposomes for enhanced cancer immunotherapy.

Autor: Zhang H; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA; Department of Biomedical Engineering, University of California, Davis, CA 95616, USA., Tang WL; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA., Kheirolomoom A; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA; Department of Biomedical Engineering, University of California, Davis, CA 95616, USA., Fite BZ; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA., Wu B; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA., Lau K; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA., Baikoghli M; Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA., Raie MN; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA., Tumbale SK; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA., Foiret J; Department of Radiology, Stanford University, Palo Alto, CA 94304, USA., Ingham ES; Department of Biomedical Engineering, University of California, Davis, CA 95616, USA., Mahakian LM; Department of Biomedical Engineering, University of California, Davis, CA 95616, USA., Tam SM; Department of Biomedical Engineering, University of California, Davis, CA 95616, USA., Cheng RH; Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA., Borowsky AD; Center for Comparative Medicine, University of California, Davis, CA 95616, USA., Ferrara KW; Molecular Imaging Program, Department of Radiology, Stanford University, 3165 Porter Drive, Palo Alto, CA 94304, USA. Electronic address: kwferrar@stanford.edu.
Jazyk: angličtina
Zdroj: Journal of controlled release : official journal of the Controlled Release Society [J Control Release] 2021 Feb 10; Vol. 330, pp. 1080-1094. Date of Electronic Publication: 2020 Nov 13.
DOI: 10.1016/j.jconrel.2020.11.013
Abstrakt: Resiquimod (R848) is a toll-like receptor 7 and 8 (TLR7/8) agonist with potent antitumor and immunostimulatory activity. However, systemic delivery of R848 is poorly tolerated because of its poor solubility in water and systemic immune activation. In order to address these limitations, we developed an intravenously-injectable formulation with R848 using thermosensitive liposomes (TSLs) as a delivery vehicle. R848 was remotely loaded into TSLs composed of DPPC: DSPC: DSPE-PEG2K (85:10:5, mol%) with 100 mM FeSO 4 as the trapping agent inside. The final R848 to lipid ratio of the optimized R848-loaded TSLs (R848-TSLs) was 0.09 (w/w), 10-fold higher than the previously-reported values. R848-TSLs released 80% of R848 within 5 min at 42 °C. These TSLs were then combined with αPD-1, an immune checkpoint inhibitor, and ultrasound-mediated hyperthermia in a neu deletion (NDL) mouse mammary carcinoma model (Her2 + , ER/PR negative). Combined with αPD-1, local injection of R848-TSLs showed superior efficacy with complete NDL tumor regression in both treated and abscopal sites achieved in 8 of 11 tumor bearing mice over 100 days. Immunohistochemistry confirmed enhanced CD8 + T cell infiltration and accumulation by R848-TSLs. Systemic delivery of R848-TSLs, combined with local hyperthermia and αPD-1, inhibited tumor growth and extended median survival from 28 days (non-treatment control) to 94 days. Upon re-challenge with reinjection of tumor cells, none of the previously cured mice developed tumors, as compared with 100% of age-matched control mice. The dose of R848 (10 μg for intra-tumoral injection or 6 mg/kg for intravenous injection delivered up to 4 times) was well-tolerated without weight loss or organ hypertrophy. In summary, we developed R848-TSLs that can be administered locally or systematically, resulting in tumor regression and enhanced survival when combined with αPD-1 in mouse models of breast cancer.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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