Detection of association of IL1β, IL4R, and IL6 gene polymorphisms with cervical cancer in the Bangladeshi women by tetra-primer ARMS-PCR method.

Autor: Muhammad SB; Department of Pharmacy, Noakhali Science and Technology University, Noakhali 3814, Bangladesh., Hassan F; Department of Pharmacy, Noakhali Science and Technology University, Noakhali 3814, Bangladesh., Bhowmik KK; Department of Pharmacy, Noakhali Science and Technology University, Noakhali 3814, Bangladesh., Millat MS; Department of Pharmacy, Noakhali Science and Technology University, Noakhali 3814, Bangladesh., Sarwar MS; Department of Pharmacy, Noakhali Science and Technology University, Noakhali 3814, Bangladesh., Aziz MA; Department of Pharmacy, Noakhali Science and Technology University, Noakhali 3814, Bangladesh., Barek MA; Department of Pharmacy, Noakhali Science and Technology University, Noakhali 3814, Bangladesh., Sarowar Uddin M; Department of Pharmacy, Noakhali Science and Technology University, Noakhali 3814, Bangladesh., Ferdous M; Department of Pharmacy, Noakhali Science and Technology University, Noakhali 3814, Bangladesh., Safiqul Islam M; Department of Pharmacy, Noakhali Science and Technology University, Noakhali 3814, Bangladesh. Electronic address: research_safiq@yahoo.com.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2021 Jan; Vol. 90, pp. 107131. Date of Electronic Publication: 2020 Nov 10.
DOI: 10.1016/j.intimp.2020.107131
Abstrakt: Background: Cervical cancer (CC) is the main cause of cancer-related deaths among women in developing countries. It is the second leading female malignancy in Bangladesh in terms of incidence and mortality. Our present study aimed to investigate the association of IL1β (rs16944), IL4R (rs1801275), and IL6 (rs1800797) gene polymorphisms with the susceptibility of cervical cancer.
Materials and Methods: This case-control study was conducted on 252 cervical cancer patients and 228 healthy volunteers, using tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR).
Results: In the case of rs16944 polymorphism, GG genotype (OR = 2.10, 95%CI = 1.24-3.56), dominant model (OR = 1.71, 95% CI = 1.11-2.63), recessive model (OR = 1.54, 95% CI = 1.01-2.35), and G allele (OR = 1.30, 95% CI = 1.005-1.68) were significantly associated with increased cervical cancer risk. Among these, GG genotype and dominant model remained significant after the Bonferroni correction (p < 0.017). For rs1801275 polymorphism, GG genotype (OR = 2.66, 95% CI = 1.49-4.75), dominant model (OR = 1.49, 95% CI = 1.04-2.14), recessive model (OR = 2.45, 95% CI = 1.40-4.27), and G allele (OR = 1.59, 95% CI = 1.21-2.10) significantly elevated the risk of cervical cancer but significance did not exist for dominant model after the Bonferroni correction. rs1800797 variant showed significantly increased risk in all genetic models including, AG genotype (OR = 8.13, 95% CI = 5.27-12.55), AA genotype (OR = 9.86, 95% CI = 2.76-35.21), dominant model (OR = 8.25, 95% CI = 5.40-12.60), recessive model (OR = 4.41, 95% CI = 1.25-15.56), and A allele (OR = 4.99, 95% CI = 3.49-7.13) and the significances were consistent with the Bonferroni correction except recessive model. Haplotyping analysis indicates that GAA (p = 5.15x10 -5 ) and GAG haplotypes (p = 4.72x10 -9 ) significantly decreased the risk of CC, whereas AAA (p = 3.89x10-9), AAG (p = 0.0003), AGA (p = 3.98x10-5) and AGG haplotypes (p = 0.002) significantly increased the risk of CC. The IL1β mRNA level was up-regulated, which was associated with poor prognosis in silico.
Conclusion: Our results conclude that rs16944 (IL1β), rs1801275 (IL4R), and rs1800797 (IL6) polymorphisms are associated with cervical cancer in Bangladeshi women.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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