Analysis of Lymphotoxin Alpha Expression in Human Retina and Generation of Expression Vectors to Functional Characterization of Polymorphisms in the Tumor Necrosis Factor Locus.

Autor: Usategui-Martín R; Instituto Universitario de Oftalmobiología Aplicada (IOBA), University of Valladolid, Valladolid, Spain. rusategui@gmail.com., Rodriguez-Hernández I; Molecular Medicine Unit, Departament of Medicine, University of Salamanca, Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain.; Instituto de Biología Molecular y Celular del Cáncer (IBMCC), University of Salamanca-CSIC, Salamanca, Spain., González-Sarmiento R; Molecular Medicine Unit, Departament of Medicine, University of Salamanca, Institute of Biomedical Research of Salamanca (IBSAL), Salamanca, Spain.; Instituto de Biología Molecular y Celular del Cáncer (IBMCC), University of Salamanca-CSIC, Salamanca, Spain., Sobas EM; Instituto Universitario de Oftalmobiología Aplicada (IOBA), University of Valladolid, Valladolid, Spain.; Nursing School, University of Valladolid, Valladolid, Spain., Pastor-Jimeno JC; Instituto Universitario de Oftalmobiología Aplicada (IOBA), University of Valladolid, Valladolid, Spain.; Departament of Ophthalmology, Hospital Clínico Universitario de Valladolid, Valladolid, Spain.; Red Temática de Investigación Cooperativa en Salud (RETICS), Oftared, Instituto de Salud Carlos III, Valladolid, Spain.; Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León, Valladolid, Spain., Pastor-Idoate S; Instituto Universitario de Oftalmobiología Aplicada (IOBA), University of Valladolid, Valladolid, Spain. pastoridoate.salvador@gmail.com.; Departament of Ophthalmology, Hospital Clínico Universitario de Valladolid, Valladolid, Spain. pastoridoate.salvador@gmail.com.; Red Temática de Investigación Cooperativa en Salud (RETICS), Oftared, Instituto de Salud Carlos III, Valladolid, Spain. pastoridoate.salvador@gmail.com.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2248, pp. 243-250.
DOI: 10.1007/978-1-0716-1130-2_18
Abstrakt: With the evolution of new genomic sequencing technologies an important amount of genomic data has been provided. As a consequence of this, many gene polymorphisms have been shown to be significantly associated with different disorders. Many strategies have been implemented to reveal the role of having more than one allele at a specific locus and their involvement in the illnesses. Site-directed mutagenesis is one of the most common strategies to understand the regulatory regions of genes and the relationship between the protein structure and its function. Here, we describe the analysis of lymphotoxin alpha expression in human retina and the generation of expression vectors to functional characterization of polymorphisms in the tumor necrosis factor locus using pCEFL-Flag expression vector and transfection assays in COS-1 cell line.
Databáze: MEDLINE
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