Generation and Evaluation of Bispecific Anti-TNF Antibodies Based on Single-Chain V H H Domains.

Autor:	Nosenko MA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Atretkhany KN; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Mokhonov VV; Blokhina Scientific Research Institute of Epidemiology of Nizhny Novgorod, Nizhny Novgorod, Russia., Chuvpilo SA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.; Sirius University of Science and Technology, Sochi, Russia., Yanvarev DV; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Drutskaya MS; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia., Tillib SV; Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia., Nedospasov SA; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. sergei.nedospasov@gmail.com.; Lomonosov Moscow State University, Moscow, Russia. sergei.nedospasov@gmail.com.; Sirius University of Science and Technology, Sochi, Russia. sergei.nedospasov@gmail.com.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2248, pp. 91-107.
DOI:	10.1007/978-1-0716-1130-2_7
Abstrakt:	Systemic cytokine inhibition may be an effective therapeutic strategy for several autoimmune diseases. However, recent studies suggest that tissue or cell type-specific targeting of certain cytokines, including TNF, may have distinct advantages and show fewer side effects. Here we describe protocols for generating and testing bispecific cytokine inhibitors using variable domain of single-chain antibodies from Camelidae (V H H) with a focus on cell-specific TNF inhibitors.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu