Molecular characterization of begomovirus-betasatellite-alphasatellite complex associated with okra enation leaf curl disease in Northern Sri Lanka.

Autor: Emmanuel CJ; Department of Botany, Faculty of Science, University of Jaffna, Jaffna, 40000 JA Sri Lanka., Manohara S; Department of Botany, Faculty of Science, University of Jaffna, Jaffna, 40000 JA Sri Lanka., Shaw MW; School of Agriculture, Policy and Development, University of Reading, Reading, RG6 6BZ UK.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2020 Dec; Vol. 10 (12), pp. 506. Date of Electronic Publication: 2020 Nov 04.
DOI: 10.1007/s13205-020-02502-z
Abstrakt: Okra enation leaf curl is a newly emerging disease in commercial okra cultivation fields in Northern Sri Lanka. The present study aimed to identify and characterize the causative begomovirus and associated satellites. Okra plants showing the enation leaf curl disease symptoms were collected from Vavuniya and Jaffna districts of Northern Province. The PCR diagnostic and genome sequencing revealed that the symptomatic okra plants are associated with begomovirus, betasatellite, and alphasatellite complex. The begomovirus isolates shared 98.2-99.7% nucleotide identity with Okra enation leaf curl virus . The betasatellites showed 96-98.8% nucleotide identity with Bhendi yellow vein mosaic betasatellite which is usually associated with Bhendi yellow vein mosaic disease. Two distinct alphasatellite species, Okra leaf curl alphasatellite and Bhendi yellow vein mosaic alphasatellite , were identified in leaf samples with enation leaf curl disease. The disease was transmitted by whiteflies from diseased plants to healthy plants. Hybrid varieties were more susceptible to the disease compared to cultivated varieties.
Competing Interests: Conflict of interestNo potential conflict of interest was reported by the authors.
(© King Abdulaziz City for Science and Technology 2020.)
Databáze: MEDLINE
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