Identification of genetic factors controlling phosphorus utilization efficiency in wheat by genome-wide association study with principal component analysis.

Autor: Safdar LB; Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan. Electronic address: luqman@bs.qau.edu.pk., Umer MJ; Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China., Almas F; Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan., Uddin S; Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan; Plant Breeding Institute, Faculty of Agriculture & Environment, University of Sydney, Australia., Safdar QT; Government College for Women, 266 RB Khurianwala, Faisalabad 37630, Pakistan., Blighe K; Institute of Ophthalmology, 11-43 Bath Street, University College London, London, UK., Quraishi UM; Department of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan. Electronic address: umasood@qau.edu.pk.
Jazyk: angličtina
Zdroj: Gene [Gene] 2021 Feb 05; Vol. 768, pp. 145301. Date of Electronic Publication: 2020 Nov 09.
DOI: 10.1016/j.gene.2020.145301
Abstrakt: Despite the economic importance of P utilization efficiency, information on genetic factors underlying this trait remains elusive. To address that, we performed a genome-wide association study in a spring wheat diversity panel ranging from landraces to elite varieties. We evaluated the phenotype variation for P utilization efficiency in controlled conditions and genotype variation using wheat 90 K SNP array. Phenotype variables were transformed into a smaller set of uncorrelated principal components that captured the most important variation data. We identified two significant loci associated with both P utilization efficiency and the 1st principal component on chromosomes 3A and 4A: qPE1-3A and qPE2-4A. Annotation of genes at these loci revealed 53 wheat genes, among which 6 were identified in significantly enriched pathways. The expression pattern of these 6 genes indicated that TraesCS4A02G481800, involved in pyruvate metabolism and TCA cycle, had a significantly higher expression in the P efficient variety under limited P conditions. Further characterization of these loci and candidate genes can help stimulate P utilization efficiency in wheat.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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