| Autor: |
Stout AE; Departments of Microbiology and Immunology, Cornell University, Ithaca, NY., Hofmar-Glennon HG; College of Veterinary Medicine, and Master of Public Health Program, Cornell University, Ithaca, NY., André NM; Departments of Microbiology and Immunology, Cornell University, Ithaca, NY., Goodman LB; Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY., Anderson RR; Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY., Mitchell PK; Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY., Thompson BS; Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY., Lejeune M; Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY., Whittaker GR; Departments of Microbiology and Immunology, Cornell University, Ithaca, NY.; College of Veterinary Medicine, and Master of Public Health Program, Cornell University, Ithaca, NY., Goodrich EL; Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY. |
| Abstrakt: |
In the United States, horses are used for a variety of purposes including recreation, exhibition, and racing. As farm, performance, and companion animals, horses are a unique species from a zoonotic disease risk perspective, and the risks of subclinical infections spreading among horses can pose challenges. Using a nanoscale real-time PCR platform, we investigated the prevalence of 14 enteric pathogens, 11 Escherichia coli genes, and 9 respiratory pathogens in fecal samples from 97 apparently healthy horses at a multi-day horse event. In addition, sugar flotation test was performed for fecal parasites. E. coli f17 was commonly detected, prevalent in 59% of horses, followed closely by Streptococcus equi subsp. zooepidemicus (55%). Additional pathogens recognized included betacoronavirus, Campylobacter jejuni, Cryptosporidium sp., E. coli O157, equine adenovirus 1, equine rhinitis B virus, and others. The use of PCR data may overestimate the true prevalence of these pathogens but provides a sensitive overview of common pathogens present in healthy horses. Our results prompt the continued need for practical biosecurity measures at horse shows, both to protect individuals interacting with these horses and to minimize transmission among horses. |
