Initial displacement of the intra-articular surface after articular fracture correlates with PTA in C57BL/6 mice but not "superhealer" MRL/MpJ mice.

Autor: Vovos TJ; Department of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina, USA., Furman BD; Department of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina, USA., Huebner JL; Duke Molecular Physiology Institute, Duke University School of Medicine, Durham, North Carolina, USA., Kimmerling KA; Department of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina, USA.; Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA., Utturkar GM; Department of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina, USA., Green CL; Department of Biostatistics and Bioinformatics, Duke University Medical Center, Durham, North Carolina, USA., Kraus VB; Duke Molecular Physiology Institute, Duke University School of Medicine, Durham, North Carolina, USA.; Department of Medicine, Duke University School of Medicine, Durham, North Carolina, USA., Guilak F; Department of Orthopaedic Surgery, Washington University in St. Louis, St. Louis, Missouri, USA.; Shriners Hospital for Children-St. Louis, St. Louis, Missouri, USA., Olson SA; Department of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina, USA.
Jazyk: angličtina
Zdroj: Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2021 Sep; Vol. 39 (9), pp. 1977-1987. Date of Electronic Publication: 2020 Dec 08.
DOI: 10.1002/jor.24912
Abstrakt: Posttraumatic arthritis (PTA) occurs commonly after articular fracture and may arise, in part, from joint surface incongruity after injury. MRL/MpJ (MRL) "super-healer" mice are protected from PTA compared to C57BL/6 (B6) mice following articular fracture. However, the relationship between the initial displacement of the articular surface, biologic response, and susceptibility to PTA after fracture remains unclear. The objective of this study was to assess whether joint incongruity after articular fracture, as measured by in vivo micro-computed tomography (microCT), could predict pathomechanisms of PTA in mice. B6 and MRL mice (n = 12/strain) received a closed articular fracture (fx) of the left tibial plateau. Articular incongruity was quantified as bone surface deviations (BSD) for each in vivo microCT scan obtained from pre-fx to 8 weeks post-fx, followed by histologic assessment of arthritis. Serum concentrations of bone formation (PINP) and bone resorption (CTX-I) biomarkers were quantified longitudinally. Both strains showed increases in surface incongruity over time, as measured by increases in BSD. In B6 mice, acute surface incongruity was significantly correlated to the severity of PTA (R 2  = 0.988; p = .0006), but not in MRL mice (R 2  = 0.224; p = .220). PINP concentrations significantly decreased immediately post-fx in B6 mice (p = .023) but not in MRL mice, indicating higher bone synthesis in MRL mice. MRL/MpJ mice demonstrate a unique biologic response to articular fracture such that the observed articular bone surface displacement does not correlate with the severity of subsequent PTA. Clinical Relevance: Identifying therapies to enhance acute biologic repair following articular fracture may mitigate the risk of articular surface displacement for PTA.
(© 2020 Orthopaedic Research Society. Published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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