Resistance profile to antimicrobials agents in methicillin-resistant Staphylococcus aureus isolated from hospitals in South Brazil between 2014-2019.

Autor:	Rossato AM; Universidade Federal de Ciências da Saúde de Porto Alegre, Programa de Pós-graduação em Ciências da Saúde, Porto Alegre, RS, Brasil., Primon-Barros M; Universidade Federal de Ciências da Saúde de Porto Alegre, Programa de Pós-graduação em Ciências da Saúde, Porto Alegre, RS, Brasil., Rocha LDL; Universidade Federal de Ciências da Saúde de Porto Alegre, Programa de Pós-graduação em Ciências da Saúde, Porto Alegre, RS, Brasil., Reiter KC; Universidade Federal de Ciências da Saúde de Porto Alegre, Programa de Pós-graduação em Ciências da Saúde, Porto Alegre, RS, Brasil., Dias CAG; Universidade Federal de Ciências da Saúde de Porto Alegre, Programa de Pós-graduação em Ciências da Saúde, Porto Alegre, RS, Brasil., d'Azevedo PA; Universidade Federal de Ciências da Saúde de Porto Alegre, Programa de Pós-graduação em Ciências da Saúde, Porto Alegre, RS, Brasil.
Jazyk:	angličtina
Zdroj:	Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2020 Nov 06; Vol. 53, pp. e20200431. Date of Electronic Publication: 2020 Nov 06 (Print Publication: 2020).
DOI:	10.1590/0037-8682-0431-2020
Abstrakt:	Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen causing healthcare-associated infections. Owing to the restricted use of beta-lactams in MRSA infections, non-beta-lactam antimicrobials are required for treatment. However, MRSA can develop resistance mechanisms to non-beta-lactam antimicrobials, which reduces viable treatment options. Here, we evaluated the antimicrobial susceptibility and resistance genes of MRSA isolated from hospitalized patients in South Brazil. Methods: The antimicrobial susceptibilities of hospital MRSA (217) isolates were determined by disk diffusion or microdilution methods. Additionally, the presence of 14 resistance genes and SCCmec typing was performed by PCR. Results: Among the antimicrobials tested, we observed high erythromycin (74.2%), ciprofloxacin (64.5%), and clindamycin (46.1%) resistance rates and complete susceptibility to linezolid and vancomycin. Seventeen different patterns of MRSA antimicrobial resistance were observed, of which 42.9% represented multidrug resistance. Among erythromycin-resistant MRSA, 53.4%, 45.3%, 37.9%, 13.0%, and 6.8% carried ermA, msrA, msrB, ermC, and ermB genes, respectively. Among clindamycin-resistant MRSA, 83%, 17%, 10%, 4%, and 2% carried ermA, ermC, ermB, linA, and linB genes, respectively. Among gentamicin-resistant MRSA, 96.8%, 83.9%, and 9.7% carried aac(6')/aph(2''), aph(3')-IIIa, and ant(4')-Ia genes, respectively. Among tetracycline-resistant MRSA, 6.5% and 93.5% carried tetK and tetM genes, respectively. Lastly, among trimethoprim/sulfamethoxazole-resistant MRSA, 13.3% and 100% carried dfrA and dfrG genes, respectively. The SCCmec type IV isolates were detected more frequently, whereas the SCCmec type III isolates exhibited higher multidrug resistance. Conclusions: The study data provides information regarding the MRSA resistance profile in South Brazil that is associated with the clinical conditions of patients and can contribute to clinical decision-making.
Databáze:	MEDLINE
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