Açaí ( Euterpe oleracea Mart.) presents anti-neuroinflammatory capacity in LPS-activated microglia cells.

Autor: de Souza DV; Graduate Program in Nanoscience, Franciscan University, Santa Maria, RS, Brazil.; Laboratory of Cell Culture and Genetics, Franciscan University, Santa Maria, RS, Brazil., Pappis L; Graduate Program of Pharmaceutical Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil., Bandeira TT; Laboratory of Cell Culture and Genetics, Franciscan University, Santa Maria, RS, Brazil., Sangoi GG; Laboratory of Cell Culture and Genetics, Franciscan University, Santa Maria, RS, Brazil., Fontana T; Laboratory of Cell Culture and Genetics, Franciscan University, Santa Maria, RS, Brazil., Rissi VB; Department of Agriculture, Biodiversity and Forests, Federal University of Santa Catarina, Curitibanos, SC, Brazil., Sagrillo MR; Graduate Program in Nanoscience, Franciscan University, Santa Maria, RS, Brazil.; Laboratory of Cell Culture and Genetics, Franciscan University, Santa Maria, RS, Brazil., Duarte MM; Graduate Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Duarte T; Graduate Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Bodenstein DF; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada., Andreazza AC; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada.; Centre for Addiction and Mental Health, Toronto, ON, Canada., Cruz IBMD; Graduate Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Ribeiro EE; Third Age Open University, University of Amazonas State, Manaus, AM, Brazil., Antoniazzi A; Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, RS, Brazil., Ourique AF; Graduate Program in Nanoscience, Franciscan University, Santa Maria, RS, Brazil., Machado AK; Graduate Program in Nanoscience, Franciscan University, Santa Maria, RS, Brazil.; Laboratory of Cell Culture and Genetics, Franciscan University, Santa Maria, RS, Brazil.
Jazyk: angličtina
Zdroj: Nutritional neuroscience [Nutr Neurosci] 2022 Jun; Vol. 25 (6), pp. 1188-1199. Date of Electronic Publication: 2020 Nov 10.
DOI: 10.1080/1028415X.2020.1842044
Abstrakt: Introduction: Neuropsychiatric diseases are responsible for one of the highest burden of morbidity and mortality worldwide. These illnesses include schizophrenia, bipolar disorder, and major depression. Individuals affected by these diseases may present mitochondrial dysfunction and oxidative stress. Additionally, patients also have increased peripheral and neural chronic inflammation. The Brazilian fruit, açaí, has been demonstrated to be a neuroprotective agent through its recovery of mitochondrial complex I activity. This extract has previously shown anti-inflammatory effects in inflammatory cells. However, there is a lack of understanding of potential anti-neuroinflammatory mechanisms, such as cell cycle involvement.
Objective: The objective of this study is to evaluate the anti-neuroinflammatory potential of an açaí extract in lipopolysaccharide-activated BV-2 microglia cells.
Methods: Açaí extract was produced and characterized through high performance liquid chromatography. Following açaí extraction and characterization, BV-2 microglia cells were activated with LPS and a dose-response curve was generated to select the most effective açaí dose to reduce cellular proliferation. This dose was then used to assess reactive oxygen species (ROS) production, double-strand DNA release, cell cycle modulation, and cytokine and caspase protein expression.
Results: Characterization of the açaí extract revealed 10 bioactive molecules. The extract reduced cellular proliferation, ROS production, and reduced pro-inflammatory cytokines and caspase 1 protein expression under 1 μg/mL in LPS-activated BV-2 microglia cells but had no effect on double strand DNA release. Additionally, açaí treatment caused cell cycle arrest, specifically within synthesis and G2/Mitosis phases.
Conclusion: These results suggest that the freeze-dried hydroalcoholic açaí extract presents high anti-neuroinflammatory potential.
Databáze: MEDLINE
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