[Effects of exogenous H 2 S on hepatic fibrosis in diabetic mice and its mechanism].

Autor: Xi YX; Departmentof Pathophysiology, Harbin Medical University, Harbin 150086, China., Wen X; Departmentof Pathophysiology, Harbin Medical University, Harbin 150086, China., Jiao LJ; Departmentof Pathophysiology, Harbin Medical University, Harbin 150086, China., Wei YX; Departmentof Pathophysiology, Harbin Medical University, Harbin 150086, China., Chang GQ; Departmentof Pathophysiology, Harbin Medical University, Harbin 150086, China., Wu R; Departmentof Pathophysiology, Harbin Medical University, Harbin 150086, China., Sun FQ; Departmentof Pathophysiology, Harbin Medical University, Harbin 150086, China., Hao JH; Departmentof Pathophysiology, Harbin Medical University, Harbin 150086, China., Li HZ; Departmentof Pathophysiology, Harbin Medical University, Harbin 150086, China.
Jazyk: čínština
Zdroj: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology [Zhongguo Ying Yong Sheng Li Xue Za Zhi] 2020 Jul; Vol. 36 (4), pp. 318-320.
DOI: 10.12047/j.cjap.5965.2020.068
Abstrakt: Objective: To investigate the effects of exogenous hydrogen sulfide (H 2 S) on the hepatic fibrosis in diabetic mice and its mechanism.
Methods: Twenty-four C57 male mice (weight 22±2 g) were randomly divided into three groups ( n =8): ① Normal control group (Control): Mice were intraperitoneally injected equal amount of normal saline, the injection time was the same as that of the experimental groups; ② Diabetes model groups (HG): Streptozotocin (STZ) was injected intraperitoneally once according to body weight (150 mg/kg) to establish diabetes model; ③ NaHS treatment groups (HG + NaHS): Mice were intraperitoneally injected with NaHS (100 μmol/L·kg·d) once a day for 12 consecutive weeks. The hepatocyte injury was detected by HE staining; the hepatic fibrosis was observed through Masson staining; the protein expressions of cystathionine - β - synthetase (CBS), collagen-I (CoL-I), collagen-III (CoL-III) and matrix metalloproteinase-9 (MMP-9) were detected by Western blot.
Results: Compared with the control group, the damage and fibrosis of hepatocyte were significantly aggravated, the expression of CBS proteins was decreased ( P ＜0.01), and the expression levels of CoL-I, CoL-III and MMP-9 proteins were increased ( P ＜0.01) in the diabetic model group. Compared with the diabetic model group, the damage and fibrosis of hepatocyte were significantly lightened, the expression of CBS proteins was obviously increased ( P ＜0.01), and the expression levels of CoL-I, CoL-III and MMP-9 proteins were markedly decreased ( P ＜ 0.01).
Conclusion: H 2 S inhibits the hepatic fibrosis in diabetic mice, and its mechanism is related to the decrease of collagen and matrix metalloproteinase-9.
Databáze: MEDLINE
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