Effect of ferrate and monochloramine disinfection on the physiological and transcriptomic response of Escherichia coli at late stationary phase.

Autor: Daer S; Department of Civil, Construction and Environmental Engineering, Iowa State University, Ames, IA, United States; Environmental Sciences Interdepartmental Graduate Program, Iowa State University, Ames, IA, United States., Goodwill JE; Department of Civil and Environmental Engineering, University of Rhode Island, United States., Ikuma K; Department of Civil, Construction and Environmental Engineering, Iowa State University, Ames, IA, United States; Environmental Sciences Interdepartmental Graduate Program, Iowa State University, Ames, IA, United States; Interdepartmental Microbiology Graduate Program, Iowa State University, Ames, IA, United States. Electronic address: kikuma@iastate.edu.
Jazyk: angličtina
Zdroj: Water research [Water Res] 2021 Feb 01; Vol. 189, pp. 116580. Date of Electronic Publication: 2020 Oct 29.
DOI: 10.1016/j.watres.2020.116580
Abstrakt: Biological mechanisms of disinfection not only vary by disinfectant but also remain not well understood. We investigated the physiological and transcriptomic response of Escherichia coli at late stationary phase to ferrate and monochloramine in amended lake water. Although ferrate and monochloramine treatments similarly reduced culturable cell concentrations by 3-log 10 , 64% and 11% of treated cells were viable following monochloramine and ferrate treatment, respectively. This observed induction of viable but non-culturable (VBNC) state following monochloramine treatment but not ferrate is attributed to slower monochloramine disinfection kinetics (by 2.8 times) compared to ferrate. Transcriptomic analysis of E. coli at 15 min of exposure revealed that 3 times as many genes related to translation and transcription were downregulated by monochloramine compared to ferrate, suggesting that monochloramine treatment may be inducing VBNC through reduced protein synthesis and metabolism. Downregulation of universal stress response genes (rpoS, uspA) was attributed to growth-related physiological stressors during late stationary phase which may have contributed to the elevated expression levels of general stress responses pre-disinfection and, subsequently, their significant downregulation post-disinfection. Both disinfectants upregulated oxidative stress response genes (trxC, grxA, soxS), although levels of upregulation were time sensitive. This work shows that bacterial inactivation responses to disinfectants is mediated by complex molecular and growth-related responses.
Competing Interests: Declaration of Competing Interest The authors declare that there are no known competing financial interests or personal relationships that could have influenced the work and findings of this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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