The impact of curcumin-graphene based nanoformulation on cellular interaction and redox-activated apoptosis: An in vitro colon cancer study.

Autor:	Al-Ani LA; Institute of Advanced Studies, Nanotechnology & Catalysis Research Centre (NANOCAT), University of Malaya, Kuala Lumpur, Malaysia., Kadir FA; Department of Anatomy and Medical Imaging, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand., Hashim NM; Department of Pharmaceutical Chemicals, Faculty of Pharmacy, University of Malaya, Kuala Lumpur, Malaysia.; Centre for Natural Products and Drug Discovery (CENAR), University of Malaya, Kuala Lumpur, Malaysia., Julkapli NM; Institute of Advanced Studies, Nanotechnology & Catalysis Research Centre (NANOCAT), University of Malaya, Kuala Lumpur, Malaysia., Seyfoddin A; Drug Delivery Research Group, Auckland University of Technology, School of Science, Auckland, New Zealand., Lu J; School of Science, Faculty of Health & Environmental Sciences, Auckland University of Technology. Auckland, New Zealand.; College of Perfume and Aroma, Shanghai Institute of Technology, Shanghai, China., AlSaadi MA; Institute of Advanced Studies, Nanotechnology & Catalysis Research Centre (NANOCAT), University of Malaya, Kuala Lumpur, Malaysia.; University of Malaya Centre for Ionic Liquids (UMCiL), University of Malaya, Kuala Lumpur, Malaysia.; National Chair of Materials Sciences and Metallurgy, University of Nizwa, Nizwa, Sultanate of Oman., Yehye WA; Institute of Advanced Studies, Nanotechnology & Catalysis Research Centre (NANOCAT), University of Malaya, Kuala Lumpur, Malaysia.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2020 Nov 02; Vol. 6 (11), pp. e05360. Date of Electronic Publication: 2020 Nov 02 (Print Publication: 2020).
DOI:	10.1016/j.heliyon.2020.e05360
Abstrakt:	Natural plants derivatives have gained enormous merits in cancer therapy applications upon formulation with nanomaterials. Curcumin, as a popular research focus has acquired such improvements surpassing its disadvantageous low bioavailability. To this point, the available research data had confirmed the importance of nanomaterial type in orienting cellular response and provoking different toxicological and death mechanisms that may range from physical membrane damage to intracellular changes. This in turn underlines the poorly studied field of nanoformulation interaction with cells as the key determinant in toxicology outcomes. In this work, curcumin-AuNPs-reduced graphene oxide nanocomposite (CAG) was implemented as a model, to study the impact on cellular membrane integrity and the possible redox changes using colon cancer in vitro cell lines (HT-29 and SW-948), representing drug-responsive and resistant subtypes. Morphological and biochemical methods of transmission electron microscopy (TEM), apoptosis assay, reactive oxygen species (ROS) and antioxidants glutathione and superoxide dismutase (GSH and SOD) levels were examined with consideration to suitable protocols and vital optimizations. TEM micrographs proved endocytic uptake with succeeding cytoplasm deposition, which unlike other nanomaterials studied previously, conserved membrane integrity allowing intracellular cytotoxic mechanism. Apoptosis was confirmed with gold-standard morphological features observed in micrographs, while redox parameters revealed a time-dependent increase in ROS accompanied with regressive GSH and SOD levels. Collectively, this work demonstrates the success of graphene as a platform for curcumin intracellular delivery and cytotoxicity, and further highlights the importance of suitable in vitro methods to be used for nanomaterial validation. Competing Interests: The authors declare no conflict of interest. (© 2020 The Authors.)
Databáze:	MEDLINE
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