Experimental infection of pigs with different doses of the African swine fever virus Armenia 07 strain by intramuscular injection and direct contact.

Autor: Yamada M; Division of Transboundary Animal Diseases, Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Kodaira, Tokyo 187-0022, Japan.; Division of Pathology and Pathophysiology, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan., Masujin K; Division of Transboundary Animal Diseases, Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Kodaira, Tokyo 187-0022, Japan., Kameyama KI; Division of Transboundary Animal Diseases, Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Kodaira, Tokyo 187-0022, Japan., Yamazoe R; Division of Transboundary Animal Diseases, Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Kodaira, Tokyo 187-0022, Japan., Kubo T; Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Yokohama, Kanagawa 235-0008, Japan., Iwata K; Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Yokohama, Kanagawa 235-0008, Japan., Tamura A; Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Yokohama, Kanagawa 235-0008, Japan., Hibi H; Laboratory Department, Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Yokohama, Kanagawa 235-0008, Japan., Shiratori T; Yamagata Prefectural Chuo Livestock Hygiene Service Center, Yamagata, Yamagata 990-2171, Japan., Koizumi S; Saitama Prefectural Chuo Livestock Hygiene Service Center, Saitama, Saitama 331-0821, Japan., Ohashi K; Osaka Livestock Hygiene Service Center, Izumisano, Osaka 598-0048, Japan., Ikezawa M; Division of Pathology and Pathophysiology, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0856, Japan., Kokuho T; Division of Transboundary Animal Diseases, Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Kodaira, Tokyo 187-0022, Japan., Yamakawa M; Division of Transboundary Animal Diseases, Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Kodaira, Tokyo 187-0022, Japan.
Jazyk: angličtina
Zdroj: The Journal of veterinary medical science [J Vet Med Sci] 2021 Jan 05; Vol. 82 (12), pp. 1835-1845. Date of Electronic Publication: 2020 Nov 06.
DOI: 10.1292/jvms.20-0378
Abstrakt: We experimentally infected pigs with the African swine fever virus (ASFV) Armenia 07 strain (genotype II) to analyze the effect of different dose injections on clinical manifestations, virus-shedding patterns, histopathology, and transmission dynamics by direct contact. Each three pigs and four pigs were injected intramuscularly with 0.1 fifty percent hemadsorbing doses (HAD 50 )/ml, 10 1 HAD 50 /ml and 10 6 HAD 50 /ml of ASFV Armenia 07 strain, respectively. Each two of three pigs injected with 0.1 HAD 50 /ml and 10 1 HAD 50 /ml died by 10 days post inoculation. All pigs had a gross lesion of splenomegaly. Perigastric and renal lymph nodes were enlarged and resembled blood clots in nine of ten pigs. It was revealed that 0.1 HAD 50 /ml of this ASFV was sufficient to infect healthy pigs by intramuscular injection and caused sub-acute lethal disease. For the transmission study, two 8-week-old pigs were injected intramuscularly with 10 3 HAD 50 /ml of the same virus. Each of the experimentally inoculated pigs was co-housed with two 8-week-old naive pigs. All contact pigs exhibited clinical manifestations at 6 or 7 days after the experimentally inoculated pigs developed pyrexia. These findings suggest that this strain may spread slowly within a herd. Histologically, lymph nodes resembled blood clots were formed by severe blood absorption and followed hemorrhage result of disruption of the lymphoid sinus filling with absorbed red blood cells. The severity of the gross and histological lesions depended on duration after infection, regardless of the difference of injection doses in this study.
Databáze: MEDLINE
Externí odkaz: