The mitochondrial isoform glutathione peroxidase 3 (OsGPX3) is involved in ABA responses in rice plants.

Autor: Paiva ALS; Departamento de Bioquímica, Universidade Federal do Ceará, Fortaleza CEP 60440900, Brazil., Passaia G; Departamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre CEP 91501-970, Brazil., Jardim-Messeder D; Departamento de Genetica, Universidade Federal do Rio de Janeiro, CEP: 21941-912, Brazil., Nogueira FCS; Departmento de Bioquímica, Instituto de Química, Universidade Federaldo Rio de Janeiro, CEP 21941-909, Brazil., Domont GB; Departmento de Bioquímica, Instituto de Química, Universidade Federaldo Rio de Janeiro, CEP 21941-909, Brazil., Margis-Pinheiro M; Departamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre CEP 91501-970, Brazil. Electronic address: marcia.margis@ufrgs.br.
Jazyk: angličtina
Zdroj: Journal of proteomics [J Proteomics] 2021 Feb 10; Vol. 232, pp. 104029. Date of Electronic Publication: 2020 Nov 05.
DOI: 10.1016/j.jprot.2020.104029
Abstrakt: Different environmental conditions can lead plants to a condition termed oxidative stress, which is characterized by a disruption in the equilibrium between the production of reactive oxygen species (ROS) and antioxidant defenses. Glutathione peroxidase (GPX), an enzyme that acts as a peroxide scavenger in different organisms, has been identified as an important component in the signaling pathway during the developmental process and in stress responses in plants and yeast. Here, we demonstrate that the mitochondrial isoform of rice (Oryza sativa L. ssp. Japonica cv. Nipponbare) OsGPX3 is induced after treatment with the phytohormone abscisic acid (ABA) and is involved in its responses and in epigenetic modifications. Plants that have been silenced for OsGPX3 (gpx3i) present substantial changes in the accumulation of proteins related to these processes. These plants also have several altered ABA responses, such as germination, ROS accumulation, stomatal closure, and dark-induced senescence. This study is the first to demonstrate that OsGPX3 plays a role in ABA signaling and corroborate that redox homeostasis enzymes can act in different and complex pathways in plant cells. SIGNIFICANCE: This work proposes the mitochondrial glutathione peroxidase (OsGPX3) as a novel ABA regulatory pathway component. Our results suggest that this antioxidant enzyme is involved in ABA-responses, highlighting the complex pathways that these proteins can participate beyond the regulation of cellular redox status.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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