MALDI-TOF MS and genomic analysis can make the difference in the clarification of canine brucellosis outbreaks.

Autor: da Silva DAV; Department of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.; Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Brendebach H; Department of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany., Grützke J; Department of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany., Dieckmann R; Department of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany., Soares RM; Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., de Lima JTR; Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Keid LB; Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil., Hofreuter D; Department of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany. Dirk.Hofreuter@bfr.bund.de., Al Dahouk S; Department of Biological Safety, German Federal Institute for Risk Assessment, Berlin, Germany.; Department of Internal Medicine, RWTH Aachen University Hospital, Aachen, Germany.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2020 Nov 06; Vol. 10 (1), pp. 19246. Date of Electronic Publication: 2020 Nov 06.
DOI: 10.1038/s41598-020-75960-3
Abstrakt: Brucellosis is one of the most common bacterial zoonoses worldwide affecting not only livestock and wildlife but also pets. Canine brucellosis is characterized by reproductive failure in dogs. Human Brucella canis infections are rarely reported but probably underestimated due to insufficient diagnostic surveillance. To improve diagnostics, we investigated dogs in a breeding kennel that showed clinical manifestations of brucellosis and revealed positive blood cultures. As an alternative to the time-consuming and hazardous classical identification procedures, a newly developed species-specific intact-cell matrix-assisted laser desorption/ionization-time of flight mass spectrometry analysis was applied, which allowed for rapid identification of B. canis and differentiation from closely related B. suis biovar 1. High-throughput sequencing and comparative genomics using single nucleotide polymorphism analysis clustered our isolates together with canine and human strains from various Central and South American countries in a distinct sub-lineage. Hence, molecular epidemiology clearly defined the outbreak cluster and demonstrated the endemic situation in South America. Our study illustrates that MALDI-TOF MS analysis using a validated in-house reference database facilitates rapid B. canis identification at species level. Additional whole genome sequencing provides more detailed outbreak information and leads to a deeper understanding of the epidemiology of canine brucellosis.
Databáze: MEDLINE
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