| Autor: |
Bernardes-Oliveira E; Post-Graduate Program in Technological Development and Innovation in Medicines, Federal University of Rio Grande do Norte, Natal, RN, 59072-970, Brazil. bio_natalrn@yahoo.com.br., de Freitas DLD; Biological Chemistry and Chemometrics, Institute of Chemistry, Federal University of Rio Grande do Norte, Natal, RN, 59072-970, Brazil., de Morais CLM; Lancashire Teaching Hospitals NHS Trust, Royal Preston Hospital, Fulwood, Preston, PR2 9HT, UK.; School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, PR1 2HE, UK., Cornetta MDCM; Januario Cicco Maternity School, Federal University of Rio Grande do Norte, Natal, RN, 59072-970, Brazil., Camargo JDAS; Januario Cicco Maternity School, Federal University of Rio Grande do Norte, Natal, RN, 59072-970, Brazil., de Lima KMG; Biological Chemistry and Chemometrics, Institute of Chemistry, Federal University of Rio Grande do Norte, Natal, RN, 59072-970, Brazil., Crispim JCO; Post-Graduate Program in Technological Development and Innovation in Medicines, Federal University of Rio Grande do Norte, Natal, RN, 59072-970, Brazil. janacrispimfre@gmail.com.; Januario Cicco Maternity School, Federal University of Rio Grande do Norte, Natal, RN, 59072-970, Brazil. janacrispimfre@gmail.com. |
| Abstrakt: |
Gestational diabetes mellitus (GDM) is a hyperglycaemic imbalance first recognized during pregnancy, and affects up to 22% of pregnancies worldwide, bringing negative maternal-fetal consequences in the short- and long-term. In order to better characterize GDM in pregnant women, 100 blood plasma samples (50 GDM and 50 healthy pregnant control group) were submitted Attenuated Total Reflection Fourier-transform infrared (ATR-FTIR) spectroscopy, using chemometric approaches, including feature selection algorithms associated with discriminant analysis, such as Linear Discriminant Analysis (LDA), Quadratic Discriminant Analysis (QDA) and Support Vector Machines (SVM), analyzed in the biofingerprint region between 1800 and 900 cm -1 followed by Savitzky-Golay smoothing, baseline correction and normalization to Amide-I band (~ 1650 cm -1 ). An initial exploratory analysis of the data by Principal Component Analysis (PCA) showed a separation tendency between the two groups, which were then classified by supervised algorithms. Overall, the results obtained by Genetic Algorithm Linear Discriminant Analysis (GA-LDA) were the most satisfactory, with an accuracy, sensitivity and specificity of 100%. The spectral features responsible for group differentiation were attributed mainly to the lipid/protein regions (1462-1747 cm -1 ). These findings demonstrate, for the first time, the potential of ATR-FTIR spectroscopy combined with multivariate analysis as a screening tool for fast and low-cost GDM detection. |
