Cell size influences inorganic carbon acquisition in artificially selected phytoplankton.

Autor: Malerba ME; School of Biological Sciences, Monash University, Clayton, Vic., 3800, Australia.; Centre of Geometric Biology, Monash University, Clayton, Vic., 3800, Australia., Marshall DJ; School of Biological Sciences, Monash University, Clayton, Vic., 3800, Australia.; Centre of Geometric Biology, Monash University, Clayton, Vic., 3800, Australia., Palacios MM; School of Biological Sciences, Monash University, Clayton, Vic., 3800, Australia.; Centre of Geometric Biology, Monash University, Clayton, Vic., 3800, Australia., Raven JA; Division of Plant Sciences, University of Dundee at the James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.; Climate Change Cluster, University of Technology, Sydney, Ultimo, NSW, 2007, Australia.; School of Biological Sciences, University of Western Australia, 35 Stirling Highway, Crawley, WA, 2009, Australia., Beardall J; School of Biological Sciences, Monash University, Clayton, Vic., 3800, Australia.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2021 Mar; Vol. 229 (5), pp. 2647-2659. Date of Electronic Publication: 2020 Dec 19.
DOI: 10.1111/nph.17068
Abstrakt: Cell size influences the rate at which phytoplankton assimilate dissolved inorganic carbon (DIC), but it is unclear whether volume-specific carbon uptake should be greater in smaller or larger cells. On the one hand, Fick's Law predicts smaller cells to have a superior diffusive CO 2 supply. On the other, larger cells may have greater scope to invest metabolic energy to upregulate active transport per unit area through CO 2 -concentrating mechanisms (CCMs). Previous studies have focused on among-species comparisons, which complicates disentangling the role of cell size from other covarying traits. In this study, we investigated the DIC assimilation of the green alga Dunaliella tertiolecta after using artificial selection to evolve a 9.3-fold difference in cell volume. We compared CO 2 affinity, external carbonic anhydrase (CA ext ), isotopic signatures (δ 13 C) and growth among size-selected lineages. Evolving cells to larger sizes led to an upregulation of CCMs that improved the DIC uptake of this species, with higher CO 2 affinity, higher CA ext and higher δ 13 C. Larger cells also achieved faster growth and higher maximum biovolume densities. We showed that evolutionary shifts in cell size can alter the efficiency of DIC uptake systems to influence the fitness of a phytoplankton species.
(© 2020 The Authors New Phytologist © 2020 New Phytologist Foundation.)
Databáze: MEDLINE
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