LC-MS/MS quantification of asymmetric dimethyl arginine and symmetric dimethyl arginine in plasma using surrogate matrix and derivatization with fluorescamine.
| Autor: | Junnotula V; Bioanalysis, Immunogenecity & Biomarkers, IVIVT, GlaxoSmithkline Pharmaceuticals, 1250 S. Collegeville Road, Collegeville, PA 19426, USA., Jones BR; Q2 Solutions, 19 Brown Road, Ithaca, NY 14850, USA., Gorman S; Clinical Biomarkers & Translational Research, Oncology Research & Development, Glaxosmithkline Pharmaceuticals, 1250 S. Collegeville Road, Collegeville, PA 19426, USA., Shen M; Q Solutions, 19 Brown Road, Ithaca, NY 14850, USA., Mulvana D; Q Solutions, 19 Brown Road, Ithaca, NY 14850, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Bioanalysis [Bioanalysis] 2020 Nov; Vol. 12 (22), pp. 1607-1619. Date of Electronic Publication: 2020 Nov 05. |
| DOI: | 10.4155/bio-2020-0223 |
| Abstrakt: | Aim: A novel LC-MS/MS method using a surrogate matrix and derivatization with fluorescamine was developed and validated for simultaneous quantification of asymmetric dimethyl arginine and symmetric dimethyl arginine. Methods & results: Asymmetric dimethyl arginine, symmetric dimethyl arginine and corresponding internal standards were extracted using protein precipitation and derivatization with fluorescamine followed by SPE. Derivatives were analyzed by turbo ion spray LC-MS/MS in the positive ion mode. Methodology was successfully transferred across multiple preclinical species and utilized in the support of several investigative studies. Conclusion: A new LC-MS/MS analytical methodology that utilizes a surrogate matrix and derivatization with fluorescamine was successfully developed and validated. |
| Databáze: | MEDLINE |
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