A combination of growth factors and cytokines alter preimplantation mouse embryo development, foetal development and gene expression profiles.

Autor:	Gurner KH; School of BioSciences, University of Melbourne, Parkville, VIC 3010, Australia., Truong TT; School of BioSciences, University of Melbourne, Parkville, VIC 3010, Australia., Harvey AJ; School of BioSciences, University of Melbourne, Parkville, VIC 3010, Australia., Gardner DK; School of BioSciences, University of Melbourne, Parkville, VIC 3010, Australia.; Melbourne IVF, East Melbourne, VIC 3002, Australia.
Jazyk:	angličtina
Zdroj:	Molecular human reproduction [Mol Hum Reprod] 2020 Dec 10; Vol. 26 (12), pp. 953-970.
DOI:	10.1093/molehr/gaaa072
Abstrakt:	Within the maternal tract, the preimplantation embryo is exposed to an array of growth factors (GFs) and cytokines, most of which are absent from culture media used in clinical IVF. Whilst the addition of individual GFs and cytokines to embryo culture media can improve preimplantation mouse embryo development, there is a lack of evidence on the combined synergistic effects of GFs and cytokines on embryo development and further foetal growth. Therefore, in this study, the effect of a combined group of GFs and cytokines on mouse preimplantation embryo development and subsequent foetal development and gene expression profiles was investigated. Supplementation of embryo culture media with an optimised combination of GFs and cytokines (0.05 ng/ml vascular endothelial GF, 1 ng/ml platelet-derived GF, 0.13 ng/ml insulin-like GF 1, 0.026 ng/ml insulin-like GF 2 and 1 ng/ml granulocyte colony-stimulating factor) had no effect on embryo morphokinetics but significantly increased trophectoderm cell number (P = 0.0002) and total cell number (P = 0.024). Treatment with this combination of GFs and cytokines also significantly increased blastocyst outgrowth area (P < 0.05) and, following embryo transfer, increased foetal weight (P = 0.027), crown-rump length (P = 0.017) and overall morphological development (P = 0.027). RNA-seq analysis of in vitro derived foetuses identified concurrent alterations to the transcriptional profiles of liver and placental tissues compared with those developed in vivo, with greater changes observed in the GF and cytokine treated group. Together these data highlight the importance of balancing the actions of such factors for the regulation of normal development and emphasise the need for further studies investigating this prior to clinical implementation. (© The Author(s) 2020. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu