Structural insights into the architecture and assembly of eukaryotic flagella.
| Autor: | Petriman NA; Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10c, DK-8000 Aarhus C, Denmark., Lorentzen E; Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10c, DK-8000 Aarhus C, Denmark. |
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| Jazyk: | angličtina |
| Zdroj: | Microbial cell (Graz, Austria) [Microb Cell] 2020 Sep 21; Vol. 7 (11), pp. 289-299. Date of Electronic Publication: 2020 Sep 21. |
| DOI: | 10.15698/mic2020.11.734 |
| Abstrakt: | Cilia and flagella are slender projections found on most eukaryotic cells including unicellular organisms such as Chlamydomonas, Trypanosoma and Tetrahymena, where they serve motility and signaling functions. The cilium is a large molecular machine consisting of hundreds of different proteins that are trafficked into the organelle to organize a repetitive microtubule-based axoneme. Several recent studies took advantage of improved cryo-EM methodology to unravel the high-resolution structures of ciliary complexes. These include the recently reported purification and structure determination of axonemal doublet microtubules from the green algae Chlamydomonas reinhardtii , which allows for the modeling of more than 30 associated protein factors to provide deep molecular insight into the architecture and repetitive nature of doublet microtubules. In addition, we will review several recent contributions that dissect the structure and function of ciliary trafficking complexes that ferry structural and signaling components between the cell body and the cilium organelle. Competing Interests: Conflict of interest: The authors declare no conflict of interest. (Copyright: © 2020 Petriman and Lorentzen.) |
| Databáze: | MEDLINE |
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