In-vitro study of monocytic THP-1 leukemia cell membrane elasticity with a single-cell microfluidic-assisted optical trapping system.

Autor:	Ombid RJL; OPTICS Research Unit, CENSER, De La Salle University (DLSU), Manila, Philippines.; Optics and Instrumentation Physics Laboratory, Physics Department, DLSU, Manila, Philippines., Oyong GG; OPTICS Research Unit, CENSER, De La Salle University (DLSU), Manila, Philippines.; Molecular Science Unit Laboratory, CENSER, DLSU, Manila, Philippines., Cabrera EC; Biology Department, DLSU, Manila, Philippines.; Molecular Science Unit Laboratory, CENSER, DLSU, Manila, Philippines., Espulgar WV; Department of Applied Physics, Graduate School of Engineering, Osaka University, Japan., Saito M; Department of Applied Physics, Graduate School of Engineering, Osaka University, Japan.; Advanced Photonics and Biosensing Open Innovation Laboratory, AIST-Osaka University, Photonics Center, Osaka University, Osaka 565-0871, Japan., Tamiya E; Advanced Photonics and Biosensing Open Innovation Laboratory, AIST-Osaka University, Photonics Center, Osaka University, Osaka 565-0871, Japan.; The Institute of Scientific and Industrial Research, Osaka University, Japan., Pobre RF; OPTICS Research Unit, CENSER, De La Salle University (DLSU), Manila, Philippines.; Optics and Instrumentation Physics Laboratory, Physics Department, DLSU, Manila, Philippines.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2020 Oct 01; Vol. 11 (10), pp. 6027-6037. Date of Electronic Publication: 2020 Oct 01 (Print Publication: 2020).
DOI:	10.1364/BOE.402526
Abstrakt:	We studied the elastic profile of monocytic THP-1 leukemia cells using a microfluidic-assisted optical trap. A 2- µ m fused silica bead was optically trapped to mechanically dent an immobilized single THP-1 monocyte sieved on a 15-µm microfluidic capture chamber. Cells treated with Zeocin and untreated cells underwent RT-qPCR analysis to determine cell apoptosis through gene expression in relation to each cell's deformation profile. Results showed that untreated cells with 43.05 ± 6.68 Pa are more elastic compared to the treated cells with 15.81 ± 2.94 Pa. THP-1 monocyte's elastic modulus is indicative of cell apoptosis shown by upregulated genes after Zeocin treatment. This study clearly showed that the developed technique can be used to distinguish between cells undergoing apoptosis and cells not undergoing apoptosis and which may apply to the study of other cells and other cell states as well. Competing Interests: The authors declare no conflicts of interest. (© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.)
Databáze:	MEDLINE
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