| Autor: |
Balhaddad AA; Imam Abdulrahman Bin Faisal University, Department of Restorative Dental Sciences, College of Dentistry, Dammam, Saudi Arabia., Ayoub HM; King Saud University, Dental Health Department, College of Applied Medical Sciences, Riyadh, Saudi Arabia.; Indiana University, School of Dentistry, Department of Biomedical and Applied Sciences., Gregory RL; Indiana University, School of Dentistry, Department of Biomedical and Applied Sciences. |
| Jazyk: |
angličtina |
| Zdroj: |
Brazilian dental journal [Braz Dent J] 2020 Sep-Oct; Vol. 31 (5), pp. 471-476. |
| DOI: |
10.1590/0103-6440202003207 |
| Abstrakt: |
Recently, Scardovia wiggsiae has been reported to be strongly associated with caries formation. This study aimed to establish an in vitro model of S. wiggsiae biofilm and to investigate the effect of nicotine on S. wiggsiae colony-forming units (CFUs) growth. S. wiggsiae biofilm was grown overnight using brain-heart infusion (BHI) broth supplemented with 5 g of yeast extract/L (BHI-YE). The overnight culture was used as an inoculum to grow S. wiggsiae biofilm on standardized enamel and dentin samples. Samples were incubated with different nicotine concentrations (0, 0.5, 1, 2, 4, 8, 16 and 32 mg/mL) for 3 days. The dissociated biofilms were diluted, spiral plated on blood agar plates, and incubated for 24 h. CFUs/mL were quantified using an automated colony counter. A two-way ANOVA was used to compare the effect of different nicotine concentrations on S. wiggsiae CFUs. This study demonstrated that S. wiggsiae biofilm could be initiated and formed in vitro. Increased CFUs was observed through 0.5-4 mg/mL and 0.5-8 mg/mL of nicotine using enamel and dentin substrates, respectively. 16 and 32 mg/mL of nicotine were determined as the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively. S. wiggsiae formed greater biofilm on enamel than dentin specimens in response to the nicotine stimulus. This study demonstrated the negative effect of smoking on increasing S. wiggsiae biofilm. Establishing S. wiggsiae biofilm in vitro may allow researchers in the future to have a better understanding of caries pathogenesis and bacterial interaction. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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