Mono-macrophage-Derived MANF Protects Against Lipopolysaccharide-Induced Acute Kidney Injury via Inhibiting Inflammation and Renal M1 Macrophages.

Autor: Hou C; School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China.; Biopharmaceutical Research Institute, Anhui Medical University, Hefei, 230032, Anhui, China., Mei Q; School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China.; Biopharmaceutical Research Institute, Anhui Medical University, Hefei, 230032, Anhui, China., Song X; School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China., Bao Q; School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China., Li X; School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China.; Biopharmaceutical Research Institute, Anhui Medical University, Hefei, 230032, Anhui, China., Wang D; School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China. wangdong@ahmu.edu.cn.; Biopharmaceutical Research Institute, Anhui Medical University, Hefei, 230032, Anhui, China. wangdong@ahmu.edu.cn., Shen Y; School of Basic Medical Sciences, Anhui Medical University, 81 Meishan Road, Hefei, 230032, Anhui, China. shenyx@ahmu.edu.cn.; Biopharmaceutical Research Institute, Anhui Medical University, Hefei, 230032, Anhui, China. shenyx@ahmu.edu.cn.
Jazyk: angličtina
Zdroj: Inflammation [Inflammation] 2021 Apr; Vol. 44 (2), pp. 693-703. Date of Electronic Publication: 2020 Nov 03.
DOI: 10.1007/s10753-020-01368-w
Abstrakt: The outburst of renal inflammatory response has been found to be a crucial cause of acute kidney injury (AKI). Attenuating the renal inflammation is an effective way for AKI treatment. Mesencephalic astrocyte-derived neurotrophic factor (MANF) has been proven to be an anti-inflammatory factor. However, the effect of MANF on renal inflammation induced by AKI is unknown. In this study, we have investigated the effect of mono-macrophage-derived MANF on AKI. We constructed the mono-macrophage-specific MANF knockout (Mø MANF -/- ) mouse and used lipopolysaccharide (LPS) to induce AKI in wild-type (WT) and Mø MANF -/- mice. With mono-macrophage-specific MANF deficiency, Mø MANF -/- mice had a lower survival rate, more severe renal injury, and higher serum level of pro-inflammatory TNF-α after AKI was induced by LPS. Also, compared with WT mice, there were more M1 macrophages in renal tissues of Mø MANF -/- mice with LPS treatment, which might be attributed to the enhanced NF-κB activation in the renal microenvironment. Our study indicates the immunoregulatory role of mono-macrophage-derived MANF in the pathophysiological process of AKI, as well as the potential clinical application of MANF for AKI treatment.
Databáze: MEDLINE
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