Exclusive enteral nutrition mediates gut microbial and metabolic changes that are associated with remission in children with Crohn's disease.

Autor:	Diederen K; Department of Pediatric Gastroenterology and Nutrition, Amsterdam UMC, Location AMC & VUmc, Amsterdam, The Netherlands.; Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Location AMC, Meibergdreef 69, 1105BK, Amsterdam, The Netherlands., Li JV; Division of Digestive Diseases, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK., Donachie GE; The Rowett Institute, University of Aberdeen, Aberdeen, UK., de Meij TG; Department of Pediatric Gastroenterology and Nutrition, Amsterdam UMC, Location AMC & VUmc, Amsterdam, The Netherlands., de Waart DR; Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Location AMC, Meibergdreef 69, 1105BK, Amsterdam, The Netherlands., Hakvoort TBM; Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Location AMC, Meibergdreef 69, 1105BK, Amsterdam, The Netherlands., Kindermann A; Department of Pediatric Gastroenterology and Nutrition, Amsterdam UMC, Location AMC & VUmc, Amsterdam, The Netherlands., Wagner J; Pathogen Genomics Group, Wellcome Sanger Institute, Hinxton, Cambridgeshire, UK., Auyeung V; Division of Digestive Diseases, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK.; Department of Surgery and Cancer, Imperial College London, London, UK., Te Velde AA; Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Location AMC, Meibergdreef 69, 1105BK, Amsterdam, The Netherlands., Heinsbroek SEM; Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Location AMC, Meibergdreef 69, 1105BK, Amsterdam, The Netherlands., Benninga MA; Department of Pediatric Gastroenterology and Nutrition, Amsterdam UMC, Location AMC & VUmc, Amsterdam, The Netherlands., Kinross J; Division of Digestive Diseases, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK.; Department of Surgery and Cancer, Imperial College London, London, UK., Walker AW; The Rowett Institute, University of Aberdeen, Aberdeen, UK., de Jonge WJ; Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Location AMC, Meibergdreef 69, 1105BK, Amsterdam, The Netherlands., Seppen J; Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Location AMC, Meibergdreef 69, 1105BK, Amsterdam, The Netherlands. j.seppen@amsterdamumc.nl.
Jazyk:	angličtina
Zdroj:	Scientific reports [Sci Rep] 2020 Nov 03; Vol. 10 (1), pp. 18879. Date of Electronic Publication: 2020 Nov 03.
DOI:	10.1038/s41598-020-75306-z
Abstrakt:	A nutritional intervention, exclusive enteral nutrition (EEN) can induce remission in patients with pediatric Crohn's disease (CD). We characterized changes in the fecal microbiota and metabolome to identify the mechanism of EEN. Feces of 43 children were collected prior, during and after EEN. Microbiota and metabolites were analyzed by 16S rRNA gene amplicon sequencing and NMR. Selected metabolites were evaluated in relevant model systems. Microbiota and metabolome of patients with CD and controls were different at all time points. Amino acids, primary bile salts, trimethylamine and cadaverine were elevated in patients with CD. Microbiota and metabolome differed between responders and non-responders prior to EEN. EEN decreased microbiota diversity and reduced amino acids, trimethylamine and cadaverine towards control levels. Patients with CD had reduced microbial metabolism of bile acids that partially normalized during EEN. Trimethylamine and cadaverine inhibited intestinal cell growth. TMA and cadaverine inhibited LPS-stimulated TNF-alpha and IL-6 secretion by primary human monocytes. A diet rich in free amino acids worsened inflammation in the DSS model of intestinal inflammation. Trimethylamine, cadaverine, bile salts and amino acids could play a role in the mechanism by which EEN induces remission. Prior to EEN, microbiota and metabolome are different between responders and non-responders.
Databáze:	MEDLINE
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