The MSC-MCF-7 Duet Playing Tumor Vasculogenesis and Angiogenesis onto the Chick Embryo Chorioallantoic Membrane.

Autor:	ComŞa Ş; Department of Microscopic Morphology/Histology, 'Victor Babeş' University of Medicine and Pharmacy, Timişoara, Romania.; Angiogenesis Research Center, 'Victor Babeş' University of Medicine and Pharmacy, Timişoara, Romania., CeauȘu AR; Department of Microscopic Morphology/Histology, 'Victor Babeş' University of Medicine and Pharmacy, Timişoara, Romania.; Angiogenesis Research Center, 'Victor Babeş' University of Medicine and Pharmacy, Timişoara, Romania., Popescu R; Department of Microscopic Morphology/Cell and Molecular Biology, 'Victor Babeş' University of Medicine and Pharmacy, Timişoara, Romania., SÂrb S; Department of Microscopic Morphology/Histology, 'Victor Babeş' University of Medicine and Pharmacy, Timişoara, Romania., CÎmpean AM; Department of Microscopic Morphology/Histology, 'Victor Babeş' University of Medicine and Pharmacy, Timişoara, Romania ancacimpean1972@yahoo.com.; Angiogenesis Research Center, 'Victor Babeş' University of Medicine and Pharmacy, Timişoara, Romania., Raica M; Department of Microscopic Morphology/Histology, 'Victor Babeş' University of Medicine and Pharmacy, Timişoara, Romania.; Angiogenesis Research Center, 'Victor Babeş' University of Medicine and Pharmacy, Timişoara, Romania.
Jazyk:	angličtina
Zdroj:	In vivo (Athens, Greece) [In Vivo] 2020 Nov-Dec; Vol. 34 (6), pp. 3315-3325.
DOI:	10.21873/invivo.12170
Abstrakt:	Background/aim: Human mesenchymal stem cells (hMSC) represent a versatile cell population, able to modulate the tumor microenvironment. Our aim was to recreate an open scene for the in vivo interaction between hMSC and the MCF-7 breast cancer cells (MCF-7), in order to enlighten the intimate involvement of hMSC in tumor vasculogenesis and angiogenesis. Materials and Methods: hMSC and MCF-7 were seeded onto the chick embryo chorioallantoic membrane (CAM) and incubated for 7 days. Consecutively, the morphology and the immunohistochemical profile of CAM were assessed. Results: Following this complex interaction, MCF-7 acquired a more aggressive phenotype, hMSC switched to a vascular precursor phenotype, while CAM underwent a major reset to an earlier stage, with hotspots of angiogenesis, vasculogenesis and hematopoiesis. Conclusion: The hallmark of this study was the establishment of a veritable in vivo experimental model of MSC involvement in tumor vasculogenesis and angiogenesis, allowing further analysis in the field. (Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
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