Pinto beans modulate the gut microbiome, augment MHC II protein, and antimicrobial peptide gene expression in mice fed a normal or western-style diet.

Autor: Ojo BA; Nutritional Sciences Department, Oklahoma State University, Stillwater, OK, USA., Lu P; Nutritional Sciences Department, Oklahoma State University, Stillwater, OK, USA., Alake SE; Nutritional Sciences Department, Oklahoma State University, Stillwater, OK, USA., Keirns B; Nutritional Sciences Department, Oklahoma State University, Stillwater, OK, USA., Anderson K; Nutritional Sciences Department, Oklahoma State University, Stillwater, OK, USA., Gallucci G; Nutritional Sciences Department, Oklahoma State University, Stillwater, OK, USA., Hart MD; Nutritional Sciences Department, Oklahoma State University, Stillwater, OK, USA., El-Rassi GD; Robert M Kerr Food and Agricultural Products Center, Oklahoma State University, Stillwater, Oklahoma, USA., Ritchey JW; Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma, USA., Chowanadisai W; Nutritional Sciences Department, Oklahoma State University, Stillwater, OK, USA., Lin D; Nutritional Sciences Department, Oklahoma State University, Stillwater, OK, USA., Clarke S; Nutritional Sciences Department, Oklahoma State University, Stillwater, OK, USA., Smith BJ; Nutritional Sciences Department, Oklahoma State University, Stillwater, OK, USA., Lucas EA; Nutritional Sciences Department, Oklahoma State University, Stillwater, OK, USA. Electronic address: edralin.a.lucas@okstate.edu.
Jazyk: angličtina
Zdroj: The Journal of nutritional biochemistry [J Nutr Biochem] 2021 Feb; Vol. 88, pp. 108543. Date of Electronic Publication: 2020 Nov 01.
DOI: 10.1016/j.jnutbio.2020.108543
Abstrakt: The onset of type 2 diabetes in obesity is associated with gut dysbiosis and a failure to confine commensal bacteria and toxins to the gut lumen while prebiotics may prevent these effects. This study evaluated the effects of pinto beans (PB) supplementation on cecal bacteria, short-chain fatty acids (SCFAs), distal ileal antigen presentation marker (major histocompatibility complex [MHC] II) and antimicrobial peptide genes during short-term high-fat, high sucrose (HFS) feeding. Six-week-old, male C57BL/6J mice were randomly assigned to four groups (n=12/group), and fed a control (C) or HFS diet with or without cooked PB (10%, wt/wt) for 30 days. Supplemental PB in both the C and HFS diets decreased the abundance of Tenericutes and the sulfate-reducing bacteria Bilophila. In contrast, PB raised the abundance of taxa within the SCFAs-producing family, Lachnospiraceae, compared to groups without PB. Consequently, fecal butyric acid was significantly higher in PB-supplemented groups compared to C and HFS groups. PB reversed the HFS-induced ablation of the distal ileal STAT3 phosphorylation, and up-regulated antimicrobial peptide genes (Reg3γ and Reg3β). Furthermore, the expression of MHC II protein was elevated in the PB supplemented groups compared to C and HFS. Tenericutes and Bilophilia negatively correlated with activated STAT3 and MHC II proteins. Finally, supplemental PB improved fasting blood glucose, glucose tolerance and suppressed TNFα and inducible nitric oxide synthase mRNA in the visceral adipose tissue. Put together, the beneficial impact of PB supplementation on the gut may be central to its potential to protect against diet-induced inflammation and impaired glucose tolerance.
(Copyright © 2020. Published by Elsevier Inc.)
Databáze: MEDLINE
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