A Transgenic Line That Reports CSF1R Protein Expression Provides a Definitive Marker for the Mouse Mononuclear Phagocyte System.

Autor:	Grabert K; The Roslin Institute, University of Edinburgh, Easter Bush, Midlothian EH259RG, United Kingdom., Sehgal A; Mater Research Institute-University of Queensland, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia., Irvine KM; Mater Research Institute-University of Queensland, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia., Wollscheid-Lengeling E; The Roslin Institute, University of Edinburgh, Easter Bush, Midlothian EH259RG, United Kingdom., Ozdemir DD; The Roslin Institute, University of Edinburgh, Easter Bush, Midlothian EH259RG, United Kingdom., Stables J; Mater Research Institute-University of Queensland, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia., Luke GA; School of Biology, University of St Andrews, North Haugh, St Andrews KY16 9ST, United Kingdom., Ryan MD; School of Biology, University of St Andrews, North Haugh, St Andrews KY16 9ST, United Kingdom., Adamson A; Faculty of Biology, Medicine and Health, School of Biological Sciences, Manchester M13 9PT, United Kingdom., Humphreys NE; Faculty of Biology, Medicine and Health, School of Biological Sciences, Manchester M13 9PT, United Kingdom., Sandrock CJ; Mater Research Institute-University of Queensland, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia., Rojo R; Escuela de Medicina y Ciencias de la Salud, Tecnologico de Monterrey, 64710 Monterrey, Mexico; and., Verkasalo VA; Centre for Inflammation Research, University of Edinburgh, Little France, Edinburgh EH16 4TJ, United Kingdom., Mueller W; Faculty of Biology, Medicine and Health, School of Biological Sciences, Manchester M13 9PT, United Kingdom., Hohenstein P; The Roslin Institute, University of Edinburgh, Easter Bush, Midlothian EH259RG, United Kingdom., Pettit AR; Mater Research Institute-University of Queensland, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia., Pridans C; Centre for Inflammation Research, University of Edinburgh, Little France, Edinburgh EH16 4TJ, United Kingdom., Hume DA; Mater Research Institute-University of Queensland, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia; david.hume@uq.edu.au.
Jazyk:	angličtina
Zdroj:	Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2020 Dec 01; Vol. 205 (11), pp. 3154-3166. Date of Electronic Publication: 2020 Nov 02.
DOI:	10.4049/jimmunol.2000835
Abstrakt:	The proliferation, differentiation, and survival of cells of the mononuclear phagocyte system (MPS; progenitors, monocytes, macrophages, and classical dendritic cells) are controlled by signals from the M-CSF receptor (CSF1R). Cells of the MPS lineage have been identified using numerous surface markers and transgenic reporters, but none is both universal and lineage restricted. In this article, we report the development and characterization of a CSF1R reporter mouse. A FusionRed (FRed) cassette was inserted in-frame with the C terminus of CSF1R, separated by a T2A-cleavable linker. The insertion had no effect of CSF1R expression or function. CSF1R-FRed was expressed in monocytes and macrophages and absent from granulocytes and lymphocytes. In bone marrow, CSF1R-FRed was absent in lineage-negative hematopoietic stem cells, arguing against a direct role for CSF1R in myeloid lineage commitment. It was highly expressed in marrow monocytes and common myeloid progenitors but significantly lower in granulocyte-macrophage progenitors. In sections of bone marrow, CSF1R-FRed was also detected in osteoclasts, CD169 + resident macrophages, and, consistent with previous mRNA analysis, in megakaryocytes. In lymphoid tissues, CSF1R-FRed highlighted diverse MPS populations, including classical dendritic cells. Whole mount imaging of nonlymphoid tissues in mice with combined CSF1R-FRed/ Csf1r -EGFP confirmed the restriction of CSF1R expression to MPS cells. The two markers highlight the remarkable abundance and regular distribution of tissue MPS cells, including novel macrophage populations within tendon and skeletal muscle and underlying the mesothelial/serosal/capsular surfaces of every major organ. The CSF1R-FRed mouse provides a novel reporter with exquisite specificity for cells of the MPS. (Copyright © 2020 by The American Association of Immunologists, Inc.)
Databáze:	MEDLINE
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