Incidence of BKV in the urine and blood samples of pediatric patients undergoing HSCT.

Autor: Yazısız H; Department of Medical Microbiology, Akdeniz University Medical Faculty, Antalya, Turkey., Uygun V; Department of Pediatric Hematology & Oncology, Medical Park Antalya Hospital, Antalya, Turkey., Çolak D; Department of Medical Microbiology, Akdeniz University Medical Faculty, Antalya, Turkey., Mutlu D; Department of Medical Microbiology, Akdeniz University Medical Faculty, Antalya, Turkey., Hazar V; Department of Pediatric Hematology & Oncology, MSG Medstar Yıldız Hospital, Antalya, Turkey., Öğünç D; Department of Medical Microbiology, Akdeniz University Medical Faculty, Antalya, Turkey., Öngüt G; Department of Medical Microbiology, Akdeniz University Medical Faculty, Antalya, Turkey., Küpesiz FT; Department of Pediatric Hematology & Oncology, Akdeniz University Medical Faculty, Antalya, Turkey.
Jazyk: angličtina
Zdroj: Pediatric transplantation [Pediatr Transplant] 2021 Mar; Vol. 25 (2), pp. e13894. Date of Electronic Publication: 2020 Nov 02.
DOI: 10.1111/petr.13894
Abstrakt: The aims were to investigate the incidence of BKV infection and the presence of HC in pediatric patients undergoing HSCT. Twenty-four children patients (M/F: 17/7) undergoing HSCT in a single center over a period of 1 year were included in the study. The presence of BKV DNA was determined by quantitative real-time PCR in plasma and urine samples at the following times: before transplantation, twice a week until engraftment time, and weekly for + 100 days. The mean age of the patients was 7.79 ± 5.03 years, the mean follow-up time was 95.6 ± 25.9 days, and the average number of samples per patient was 15.8 ± 3.2. BKV DNA was detected in at least one urine sample in 91.6% (n: 22) and at least one plasma sample in 75% (n:18) of the patients. The median time to the first BKV DNA positivity in urine and plasma samples was 11 (range: 1-80) and 32 days (range: 2-79), respectively. The median value of BKV DNA copies in urine and plasma were 1.7 × 10 6 (range: 2.8 × 10 1 -1.2 × 10 14 ) and 1.9 × 10 3 copies/mL (range: 3-2.1 × 10 6 ), respectively. Thirteen patients (54.2%) had hematuria with BKV viruria; 8 (33.3%) patients had viremia. The median value of the BKV DNA copies in urine and plasma was 4.4 × 10 7 (range: 65-1 × 10 11 ) and 2.9 × 10 3 (range: 7-7.8 × 10 4 ) copies/mL in these patients. Two (15.4%) of the 13 patients with BKV viruria and hematuria were diagnosed with BKV-related HC. BKV DNA viral load monitoring of urine and plasma in pediatric HSCT patients with a high risk for viral infections is valuable for understanding the development of BKV-related HC.
(© 2020 Wiley Periodicals LLC.)
Databáze: MEDLINE
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