Penetration of Carbon Nanotubes into the Retinoblastoma Tumor after Intravitreal Injection in LH BETA T AG Transgenic Mice Reti-noblastoma Model.

Autor:	Demirci H; Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan Ann Arbor, MI, USA., Wang Y; Biointerfaces Institute, University of Michigan Ann Arbor, MI, USA.; Department of Chemical Engineering, University of Michigan Ann Arbor, MI, USA., Li Q; Electrical Engineering and Computer Science, University of Michigan Ann Arbor, MI, USA., Lin CM; Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan Ann Arbor, MI, USA., Kotov NA; Biointerfaces Institute, University of Michigan Ann Arbor, MI, USA.; Department of Chemical Engineering, University of Michigan Ann Arbor, MI, USA.; Electrical Engineering and Computer Science, University of Michigan Ann Arbor, MI, USA.; Department of Biomedical Engineering, University of Michigan Ann Arbor, MI, USA.; Department of Materials Science and Engineering, University of Michigan Ann Arbor, MI, USA.; Department of Macromolecular Science and Engineering, University of Michigan Ann Arbor, MI, USA.; Michigan Center for Integrative Research in Critical Care, University of Michigan Ann Arbor, MI, USA.; Michigan Institute of Translational Nanotechnology, University of Michigan Ann Arbor, MI, USA., Grisolia ABD; Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan Ann Arbor, MI, USA., Guo JL; Electrical Engineering and Computer Science, University of Michigan Ann Arbor, MI, USA.
Jazyk:	angličtina
Zdroj:	Journal of ophthalmic & vision research [J Ophthalmic Vis Res] 2020 Oct 25; Vol. 15 (4), pp. 446-452. Date of Electronic Publication: 2020 Oct 25 (Print Publication: 2020).
DOI:	10.18502/jovr.v15i4.7778
Abstrakt:	Purpose: To evaluate the penetration of carbon nanotubes (CNTs) throughout retinoblastoma in a transgenic mice model. Methods: CNTs functionalized with fluorescein isothiocyanate and targeting ligands biotin (CTN-FITC-Bio, 0.5mg/ml), or folic acid (CNT-FITC-FA, 0.5mg/ml) were injected into the vitreous of one eye of LH BETA T AG transgenic mice. Other eye did not receive any injection and was used as control. Three mice were sacrificed at days 1, 2, and 3. Eyes were enucleated and stained with 4,6-diamidino-2-phenylindole. The sections were imaged by fluorescent microscope. The images were transformed into grey-scale in MATLAB for intensity analysis. Background intensity was normalized by marking squares outside the eyeball and using the mean intensity of these squares. Fluorescent intensity (FI) for each image was measured by calculating the intensity of a same-sized square within retinoblastoma. Results: Nine eyes of nine mice were included in each CNT-FITC-Bio and CNT-FITC-FA groups. The mean FI in CNT-FITC-Bio was 52.08 ± 6.33, 53.62 ± 9.00, and 65.54 ± 5.14 in days 1, 2, and 3, respectively. The mean FI in CNT-FITC-FA was 50.28 ± 7.37, 59.21 ± 6.43, and 58.38 ± 2.32 on days 1, 2, and 3, respectively. FI was significantly higher in eyes injected with CNT-FITC-Bio and CNT-FITC-FA compared to the control eyes ( P = 0.02). There was no difference in FI between eyes with CNT-FITC-Bio and CNT-FITC-FA, and FI remained stable on days 1-3 in CNT-FITC-Bio, CNT-FITC-FA, and control eyes ( P > 0.05). Conclusion: We observed higher FI in eyes with CNT-FITC-Bio and CNT-FITC-FA compared to control eyes, showing penetration of CNTs throughout retinoblastoma. CNTs can be a carrier candidate for imaging or therapeutic purposes in retinoblastoma. Competing Interests: There are no conflicts of interest. (Copyright © 2020 Demirci et al.)
Databáze:	MEDLINE
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