The in vitro cultivation of P. falciparum ookinetes, and their enrichment on Nycodenz density gradients.

Autor: Carter EH; Department of Pure and Applied Biology, Imperial College, London., Suhrbier A, Beckers PJ, Sinden RE
Jazyk: angličtina
Zdroj: Parasitology [Parasitology] 1987 Aug; Vol. 95 ( Pt 1), pp. 25-30.
DOI: 10.1017/s0031182000057516
Abstrakt: Employing a simple method of growing ookinetes of Plasmodium falciparum in culture, 40% of mature gametocytes convert to macrogametes, 4% reach the retort-form ookinete stage and 0.45% become mature ookinetes. A single-step gradient centrifugation method on 12.5% Nycodenz enriches both gametocytes and ookinetes.
Databáze: MEDLINE