A simplified method for producing human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells.

Autor: Dewi CU; School of Medicine, Western Sydney University, Campbelltown, NSW, 2560, Australia., Mason M; School of Medicine, Western Sydney University, Campbelltown, NSW, 2560, Australia., Cohen-Hyams T; Electron Microscopy Laboratory, NSW Health Pathology and Correlative Microscopy Facility, Ingham Institute, Liverpool, NSW, 2170, Australia., Killingsworth MC; Electron Microscopy Laboratory, NSW Health Pathology and Correlative Microscopy Facility, Ingham Institute, Liverpool, NSW, 2170, Australia., Harman DG; School of Medicine, Western Sydney University, Campbelltown, NSW, 2560, Australia., Gnanasambandapillai V; Garvan-Weizmann Centre for Cellular Genomics, Darlinghurst, NSW, 2010, Australia., Liyanage L; School of Computer, Data and Mathematical Sciences, Western Sydney University, Campbelltown, NSW, 2560, Australia., O'Connor MD; School of Medicine, Western Sydney University, Campbelltown, NSW, 2560, Australia. Electronic address: m.oconnor@westernsydney.edu.au.
Jazyk: angličtina
Zdroj: Experimental eye research [Exp Eye Res] 2021 Jan; Vol. 202, pp. 108317. Date of Electronic Publication: 2020 Oct 29.
DOI: 10.1016/j.exer.2020.108317
Abstrakt: Here we describe a modified method for harvesting tens-of-millions of human lens epithelial-like cells from differentiated pluripotent stem cell cultures. To assess the utility of this method, we analysed the lens cell population via: light microscopy; single cell RNA-sequencing and gene ontology analyses; formation of light-focusing micro-lenses; mass spectrometry; and electron microscopy. Both individually and collectively, the data indicate this simplified harvesting method provides a large-scale source of stem cell-derived lens cells and micro-lenses for investigating human lens and cataract formation.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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