Th22 response induced by Mycobacterium tuberculosis strains is closely related to severity of pulmonary lesions and bacillary load in patients with multi-drug-resistant tuberculosis.

Autor: Imperiale BR; Institute of Experimental Medicine (IMEX)-CONICET, National Academy of Medicine, Buenos Aires City, Argentina., García A; Dr. F.J. Muñiz Hospital, Buenos Aires City, Argentina., Minotti A; Institute of Experimental Medicine (IMEX)-CONICET, National Academy of Medicine, Buenos Aires City, Argentina., González Montaner P; Dr. F.J. Muñiz Hospital, Buenos Aires City, Argentina.; Vaccareza Institute, UBA, Buenos Aires City, Argentina., Moracho L; Dr. F.J. Muñiz Hospital, Buenos Aires City, Argentina., Morcillo NS; Tuberculosis and Mycobacterioses Laboratory, Dr. Antonio A. Cetrángolo Hospital, Buenos Aires Province, Argentina., Palmero DJ; Dr. F.J. Muñiz Hospital, Buenos Aires City, Argentina.; Vaccareza Institute, UBA, Buenos Aires City, Argentina., Sasiain MDC; Institute of Experimental Medicine (IMEX)-CONICET, National Academy of Medicine, Buenos Aires City, Argentina., de la Barrera S; Institute of Experimental Medicine (IMEX)-CONICET, National Academy of Medicine, Buenos Aires City, Argentina.
Jazyk: angličtina
Zdroj: Clinical and experimental immunology [Clin Exp Immunol] 2021 Feb; Vol. 203 (2), pp. 267-280. Date of Electronic Publication: 2020 Nov 18.
DOI: 10.1111/cei.13544
Abstrakt: The role of interleukin-22 (IL-22) in the pathogenesis or tissue repair in human tuberculosis (TB) remains to be established. Here, we aimed to explore the ex-vivo and in-vitro T helper 22 (Th22) response in TB patients and healthy donors (HD) induced by different local multi-drug-resistant (MDR) Mvcobacterium tuberculosis (Mtb) strains. For this purpose, peripheral blood mononuclear cells from drug-susceptible (S-TB) MDR-TB patients and HD were stimulated with local MDR strains and the laboratory strain H37Rv. IL-22 and IL-17 expression and senescent status were assessed in CD4 + and CD8 + cells by flow cytometry, while IL-22 amount was measured in plasma and culture supernatants by enzyme-linked immunosorbent assay (ELISA). We found lower IL-22 amounts in plasma from TB patients than HD, together with a decrease in the number of circulating T cells expressing IL-22. In a similar manner, all Mtb strains enhanced IL-22 secretion and expanded IL-22 + cells within CD4 + and CD8 + subsets, being the highest levels detected in S-TB patients. In MDR-TB, low systemic and Mtb-induced Th22 responses associated with high sputum bacillary load and bilateralism of lung lesions, suggesting that Th22 response could be influencing the ability of MDR-TB patients to control bacillary growth and tissue damage. In addition, in MDR-TB patients we observed that the higher the percentage of IL-22 + cells, the lower the proportion of programmed cell death 1 (PD-1) + or CD57 + T cells. Furthermore, the highest proportion of senescent T cells was associated with severe lung lesions and bacillary load. Thus, T cell senescence would markedly influence Th22 response mounted by MDR-TB patients.
(© 2020 British Society for Immunology.)
Databáze: MEDLINE
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