| Autor: |
Williams S; Astrea Bioseparations Ltd, Cambridge, UK., Morton P; Albumedix Ltd. Head Office, Nottingham, UK., Baines D; Astrea Bioseparations Ltd, Cambridge, UK. d.baines@astrea-bio.com. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2178, pp. 133-148. |
| DOI: |
10.1007/978-1-0716-0775-6_11 |
| Abstrakt: |
Synthetic ligand affinity adsorbents offer an efficient means for purification of biopharmaceuticals. Single-isomer textile dye C.I. Reactive Blue and newer ligands developed by rational design and screening of chemical combinatorial libraries based on a triazine scaffold are routinely used for the capture and purification of these proteins from engineered recombinant expression systems. Here, we describe methods for the purification of recombinant human serum albumin and related fusion proteins using synthetic ligand affinity adsorbents. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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