The potential application of gold-apoferritin nanocages conjugated with 2-amino-2-deoxy-glucose for imaging of breast cancer cells.

Autor: Aslan TN; Department of Molecular Biology and Genetics, Faculty of Science, Necmettin Erbakan University, Konya, 42090, Turkey., Aşık E; Department of Biotechnology, Middle East Technical University, Ankara, 06800, Turkey.; Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA., Güray NT; Department of Biotechnology, Middle East Technical University, Ankara, 06800, Turkey.; Department of Biological Sciences, Middle East Technical University, Ankara, 06800, Turkey., Volkan M; Department of Chemistry, Middle East Technical University, Ankara, 06800, Turkey. murvet@metu.edu.tr.; Department of Micro and Nanotechnology, Middle East Technical University, Ankara, 06800, Turkey. murvet@metu.edu.tr.
Jazyk: angličtina
Zdroj: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry [J Biol Inorg Chem] 2020 Dec; Vol. 25 (8), pp. 1139-1152. Date of Electronic Publication: 2020 Oct 31.
DOI: 10.1007/s00775-020-01830-y
Abstrakt: Development of biocompatible and multifunctional nanoprobes for tumor targeting, imaging, and therapy still remains a great challenge. Herein, gold nanoparticles (AuNPs) were synthesized in the cavity of horse spleen apoferritin protein (HoSAF) and protein surface was labeled with 2-amino-2-deoxy-glucose (2DG) as a cell surface glucose transport protein specific targeting probe to study the feasibility of its usage as a computer tomography (CT) contrast agent with tumor targeting capability through in vitro experiments. 2DG conjugated and gold-loaded apoferritin (Au-HoSAF-2DG) nanoparticles (NPs) showed selective targeting for human breast adenocarcinoma (MCF-7) cells when compared to normal breast (MCF-10A) cells. This AuNP-based imaging agent was found to be non-cytotoxic in a given concentration range with an apoptotic effect upon longer exposure times towards MCF-7 cells, while MCF-10A cells were affected less. This selective cell death would also be useful for further cancer treatments with the ability of X-ray attenuation in in vitro X-ray and computed tomography (CT) imaging.
Databáze: MEDLINE
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