Papain immobilization on heterofunctional membrane bacterial cellulose as a potential strategy for the debridement of skin wounds.

Autor: Vasconcelos NF; Universidade Federal do Ceará (UFC), Departamento de Química Orgânica e Inorgânica, Bloco 935, 60455-760 Fortaleza, Ceará, Brazil. Electronic address: niedjafittipaldi@hotmail.com., Cunha AP; Universidade Federal do Ceará (UFC), Departamento de Química Orgânica e Inorgânica, Bloco 935, 60455-760 Fortaleza, Ceará, Brazil. Electronic address: arcelinapacheco@yahoo.com.br., Ricardo NMPS; Universidade Federal do Ceará (UFC), Departamento de Química Orgânica e Inorgânica, Bloco 935, 60455-760 Fortaleza, Ceará, Brazil. Electronic address: naricard@dqoi.ufc.br., Freire RS; Universidade Federal do Ceará (UFC), Central Analítica, Bloco 928, 60455-970 Fortaleza, Ceará, Brazil. Electronic address: rosefreire@fisica.ufc.br., Vieira LAP; Embrapa Agroindústria Tropical - CNPAT, Rua Dra Sara Mesquita 2270, Planalto do Pici 60511-110, Fortaleza, Ceará, Brazil. Electronic address: lidia_vieira@uol.com.br., Brígida AIS; Embrapa Agroindústria Tropical - CNPAT, Rua Dra Sara Mesquita 2270, Planalto do Pici 60511-110, Fortaleza, Ceará, Brazil. Electronic address: ana.iraidy@embrapa.br., Borges MF; Embrapa Agroindústria Tropical - CNPAT, Rua Dra Sara Mesquita 2270, Planalto do Pici 60511-110, Fortaleza, Ceará, Brazil. Electronic address: maria.fatima@embrapa.br., Rosa MF; Embrapa Agroindústria Tropical - CNPAT, Rua Dra Sara Mesquita 2270, Planalto do Pici 60511-110, Fortaleza, Ceará, Brazil. Electronic address: morsyleide.rosa@embrapa.br., Vieira RS; Universidade Federal do Ceará (UFC), Departamento de Engenharia Química, Bloco 709, 60455-760 Fortaleza, Ceará, Brazil. Electronic address: rodrigo@gpsa.ufc.br., Andrade FK; Universidade Federal do Ceará (UFC), Departamento de Engenharia Química, Bloco 709, 60455-760 Fortaleza, Ceará, Brazil. Electronic address: fabiakarine@gmail.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2020 Dec 15; Vol. 165 (Pt B), pp. 3065-3077. Date of Electronic Publication: 2020 Oct 28.
DOI: 10.1016/j.ijbiomac.2020.10.200
Abstrakt: We combined the chemical and physical methods of papain immobilization through the aldehyde groups available on oxidized bacterial cellulose (OxBC) to provide high proteolytic activity for future applications as bioactive dressing. Bacterial cellulose (BC) was obtained by the fermentation of Komagataeibacter hansenii in Hestrin-Schramm medium for 5 days, followed by purification and oxidation using NaIO 4 . Surface response methodology was used to optimize papain immobilization (2%, w/v) for 24 h. The independent variables: pH (3-7) and temperature (5 to 45 °C) were investigated. The mathematically validated optimal conditions of 45 °C and pH 7 had a statistical effect on the immobilization yield (IY) of papain in OxBC (52.9%). These ideal conditions were also used for papain immobilization in BC (unoxidized). The IY of 9.1% was lower than that of OxBC. OxBC-Papain and BC-Papain were investigated using thermal analysis, confocal microscopy, and diffusion testing. The OxBC support exhibited a more interactive chemical structure than the BC support, and was capable of immobilizing papain by covalent bonds (-C-NHR) and adsorption (ion exchange), with 93.3% recovered activity, 49.4% immobilization efficiency, and better thermal stability. Papain immobilized to OxBC by adsorption displayed 53% widespread papain activity. The results indicate the potential of prolonged bioactivity in debrided chronic wounds.
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest and that the manuscript has not been published elsewhere, is not under editorial review for publication elsewhere, and is not being submitted simultaneously to another journal.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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